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Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes

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dc.contributor.author Kalogeris, E en
dc.contributor.author Christakopoulos, P en
dc.contributor.author Katapodis, P en
dc.contributor.author Alexiou, A en
dc.contributor.author Vlachou, S en
dc.contributor.author Kekos, D en
dc.contributor.author Macris, BJ en
dc.date.accessioned 2014-03-01T01:19:26Z
dc.date.available 2014-03-01T01:19:26Z
dc.date.issued 2003 en
dc.identifier.issn 0032-9592 en
dc.identifier.uri http://hdl.handle.net/123456789/15498
dc.subject β-glucosidase en
dc.subject Cellulose hydrolysis en
dc.subject Endoglucanase en
dc.subject Solid state cultivation en
dc.subject Thermoascus aurantiacus en
dc.subject Thermophilic fungus en
dc.subject.classification Biochemistry & Molecular Biology en
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.classification Engineering, Chemical en
dc.subject.other Fungi en
dc.subject.other Thermoascus aurantiacus en
dc.title Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0032-9592(02)00242-X en
heal.identifier.secondary http://dx.doi.org/10.1016/S0032-9592(02)00242-X en
heal.language English en
heal.publicationDate 2003 en
heal.abstract Extracellular cellulolytic enzymes were produced under solid state cultivation by the thermophilic fungus Thermoascus aurantiacus and characterized. Elevated levels of endoglucanase and beta-glucosidase activities were produced simultaneously by optimization of growth factors. Under optimal growth conditions, 1572 U endoglucanase and 101.6 U beta-glucosidase per g of carbon source were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl-beta-glycosides of glucose (MUG) and cellobiose (MUG(2)) were used to characterize the cellulolytic multienzyme components after separation by isoelectric focusing. The zymogram indicated one endoglucanase and one beta-glucosidase with pI values 3.5 and 3.9, respectively. Both enzymes exhibited significant thermostability, with half-lives of 42 and 18 min, respectively, at 80 degreesC. (C) 2002 Elsevier Science Ltd. All rights reserved. en
heal.publisher ELSEVIER SCI LTD en
heal.journalName Process Biochemistry en
dc.identifier.doi 10.1016/S0032-9592(02)00242-X en
dc.identifier.isi ISI:000183135000015 en
dc.identifier.volume 38 en
dc.identifier.issue 7 en
dc.identifier.spage 1099 en
dc.identifier.epage 1104 en


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