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Production of β-fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides

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dc.contributor.author Katapodis, P en
dc.contributor.author Kalogeris, E en
dc.contributor.author Kekos, D en
dc.contributor.author Macris, BJ en
dc.contributor.author Christakopoulos, P en
dc.date.accessioned 2014-03-01T01:19:27Z
dc.date.available 2014-03-01T01:19:27Z
dc.date.issued 2003 en
dc.identifier.issn 0890-5436 en
dc.identifier.uri http://hdl.handle.net/123456789/15501
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.classification Food Science & Technology en
dc.subject.other Catalyst activity en
dc.subject.other Fungi en
dc.subject.other Hydrolysis en
dc.subject.other pH effects en
dc.subject.other Sugar (sucrose) en
dc.subject.other Synthesis (chemical) en
dc.subject.other Thermophilic fungi en
dc.subject.other Enzymes en
dc.subject.other Corynascus heterothallicus en
dc.subject.other Fungi en
dc.subject.other Sporothrix en
dc.subject.other Sporotrichum en
dc.title Production of β-fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides en
heal.type journalArticle en
heal.identifier.primary 10.1081/FBT-120019980 en
heal.identifier.secondary http://dx.doi.org/10.1081/FBT-120019980 en
heal.language English en
heal.publicationDate 2003 en
heal.abstract Production of beta-fructofuranosidase from the thermophilic fungus Sporotrichum thermophile was studied. The effect of nitrogen source, as well as the type and concentration of carbon source on enzyme production was examined. The results from flask experiments were used for the production of the enzyme in 7-1 bioreactors. beta-Fructofuranosidase from Sporotrichum thermophile showed both transfructosylating and hydrolytic activities. It was optimally, active at 60degreesC, while the optimal pHs for hydrolysis and transfructosylation were 4.0 and 6.0, respectively. Synthesis of fructooligosaccharides was maximized at 20% (w/v) initial sucrose concentration. The major sugar produced by the transfructosylating activity of the enzyme was 6-kestose. en
heal.publisher MARCEL DEKKER INC en
heal.journalName Food Biotechnology en
dc.identifier.doi 10.1081/FBT-120019980 en
dc.identifier.isi ISI:000182489300001 en
dc.identifier.volume 17 en
dc.identifier.issue 1 en
dc.identifier.spage 1 en
dc.identifier.epage 14 en


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