Optimization of xylanase production by Thermomyces lanuginosus in tomato seed meal using response surface methodology

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dc.contributor.author Katapodis, P en
dc.contributor.author Christakopoulou, V en
dc.contributor.author Christakopoulos, P en
dc.date.accessioned 2014-03-01T01:24:49Z
dc.date.available 2014-03-01T01:24:49Z
dc.date.issued 2006 en
dc.identifier.issn 0959-3993 en
dc.identifier.uri http://hdl.handle.net/123456789/17450
dc.subject Response surface method en
dc.subject Thermomyces lanuginosus en
dc.subject Tomato seed meal en
dc.subject Waste utilization en
dc.subject Xylanase en
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.other Bioreactors en
dc.subject.other Enzyme kinetics en
dc.subject.other Meats en
dc.subject.other Optimization en
dc.subject.other Seed en
dc.subject.other Yeast en
dc.subject.other Response surface method en
dc.subject.other Thermomyces lanuginosus en
dc.subject.other Tomato seed meal en
dc.subject.other Xylanase en
dc.subject.other Crops en
dc.subject.other Enzymes en
dc.subject.other Tomatoes en
dc.subject.other Yeasts en
dc.subject.other Lycopersicon esculentum en
dc.subject.other Thermomyces lanuginosus en
dc.subject.other Zea mays en
dc.title Optimization of xylanase production by Thermomyces lanuginosus in tomato seed meal using response surface methodology en
heal.type journalArticle en
heal.identifier.primary 10.1007/s11274-005-9063-7 en
heal.identifier.secondary http://dx.doi.org/10.1007/s11274-005-9063-7 en
heal.language English en
heal.publicationDate 2006 en
heal.abstract A 32 central composite experimental design was performed with the aim of optimizing xylanase production by Thermomyces lanuginosus grown on corn cobs in submerged cultures. Xylanase production was first tested on different nitrogen sources (tomato skin, tomato seed meal, corn steep liquor, meat peptone, bacto-tryptone and yeast extract). Tomato seed meal was the selected substrate to test the effect of two variables on xylanase production (corn cobs and tomato seed meal concentrations). A second-order quadratic model and a response surface method showed that the optimum condition for xylanase production was corn cobs 4.6% (w/v) and tomato seed meal 2.1% (w/v). The optimum conditions found were transferred to 7-1 bioreactors, where activities as high as 1630 U/ml were obtained. © Springer 2006. en
heal.publisher SPRINGER en
heal.journalName World Journal of Microbiology and Biotechnology en
dc.identifier.doi 10.1007/s11274-005-9063-7 en
dc.identifier.isi ISI:000237468600014 en
dc.identifier.volume 22 en
dc.identifier.issue 5 en
dc.identifier.spage 501 en
dc.identifier.epage 506 en

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