Epitope mapping of the Ro/SSA60KD autoantigen reveals disease-specific antibody-binding profiles

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dc.contributor.author Routsias, JG en
dc.contributor.author Tzioufas, AG en
dc.contributor.author SakarellosDaitsiotis, M en
dc.contributor.author Sakarellos, C en
dc.contributor.author Moutsopoulos, HM en
dc.date.accessioned 2014-03-01T01:45:07Z
dc.date.available 2014-03-01T01:45:07Z
dc.date.issued 1996 en
dc.identifier.issn 0014-2972 en
dc.identifier.uri http://hdl.handle.net/123456789/24548
dc.subject antiRo/SSA en
dc.subject epitope mapping en
dc.subject molecular mimicry en
dc.subject Sjogren's syndrome en
dc.subject systemic lupus erythematosus en
dc.subject.classification Medicine, General & Internal en
dc.subject.classification Medicine, Research & Experimental en
dc.subject.other RO RIBONUCLEOPROTEIN en
dc.subject.other AUTOIMMUNE-DISEASE en
dc.subject.other SYNTHETIC PEPTIDES en
dc.subject.other ANTIGENIC REGIONS en
dc.subject.other SJOGRENS-SYNDROME en
dc.subject.other PROTEINS en
dc.subject.other AUTOANTIBODIES en
dc.subject.other PREDICTION en
dc.subject.other ACID en
dc.title Epitope mapping of the Ro/SSA60KD autoantigen reveals disease-specific antibody-binding profiles en
heal.type journalArticle en
heal.language English en
heal.publicationDate 1996 en
heal.abstract Anti-Ro60KD autoantibodies are commonly found in sera from patients with primary Sjogren's syndrome (SS) and systemic lupus erythematosus (SLE). In order to identify the epitopes of this autoantigen, 22-mer, synthetic peptides overlapping by eight residues, and covering the entire sequence of the Ro60KD autoantigen were prepared. Three groups of sera were evaluated according to their autoantibody specificities. The first group consisted of monospecific anti Ro60KD sera from four patients with SLE and one with SS, the second one was composed of anti-Ro60KD + anti-La(SSB)-positive sera from four patients with SS and the third group included three normal sera and one anti Ro52KD serum. It was found that sera from SLE patients interact with a common antigenic site spanning the sequence TKYKQRNGWSHKDLLRSHLKP (169-190) of the Ro60KD protein. On the other hand, sera from SS patients recognise the ELYKEKALSVETEKLLKYLEAV (211-232) region of this autoantigen. Determination of the minimal required peptide length for optimal antibody recognition showed that the defined epitopes can be shortened to the NGWSHKDLLR (175-184) and KALSVETEKLLKYLEAV (216-332) sequences respectively. Inhibition experiments using the Ro60KD antigen and soluble peptides corresponding to the 175-184 and 216-232 segments further confirmed the specific antibody binding. These results, although only a small number of sera were used, indicate that the Ro60KD autoantigen, which is not characterized by disease specificity, contains two discrete epitopes specifically recognized from SLE and SS patient sera. Finally, the sequence similarity of the NGWSHKDLLR (175-184) epitope with some of the HLA haplotypes, associated with anti-lie response, deserves to be noted. en
heal.publisher BLACKWELL SCIENCE LTD en
dc.identifier.isi ISI:A1996UV06100015 en
dc.identifier.volume 26 en
dc.identifier.issue 6 en
dc.identifier.spage 514 en
dc.identifier.epage 521 en

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