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Prevalence and characterization of class 1 integrons in escherichia coli of poultry and human origin

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dc.contributor.author Vasilakopoulou, A en
dc.contributor.author Psichogiou, M en
dc.contributor.author Tzouvelekis, L en
dc.contributor.author Tassios, PT en
dc.contributor.author Kosmidis, C en
dc.contributor.author Petrikkos, G en
dc.contributor.author Roma, ES en
dc.contributor.author Charvalos, E en
dc.contributor.author Passiotou, M en
dc.contributor.author Avlami, A en
dc.contributor.author Daikos, GL en
dc.date.accessioned 2014-03-01T01:58:48Z
dc.date.available 2014-03-01T01:58:48Z
dc.date.issued 2009 en
dc.identifier.issn 15353141 en
dc.identifier.uri http://hdl.handle.net/123456789/28733
dc.subject.other amikacin en
dc.subject.other ampicillin en
dc.subject.other antibiotic agent en
dc.subject.other antiinfective agent en
dc.subject.other aztreonam en
dc.subject.other cefalotin en
dc.subject.other cefepime en
dc.subject.other cefotaxime en
dc.subject.other cefoxitin en
dc.subject.other cefuroxime en
dc.subject.other chloramphenicol en
dc.subject.other ciprofloxacin en
dc.subject.other cotrimoxazole en
dc.subject.other genomic DNA en
dc.subject.other gentamicin en
dc.subject.other imipenem en
dc.subject.other nalidixic acid en
dc.subject.other piperacillin plus tazobactam en
dc.subject.other streptomycin en
dc.subject.other tetracycline en
dc.subject.other tobramycin en
dc.subject.other animal tissue en
dc.subject.other article en
dc.subject.other controlled study en
dc.subject.other disk diffusion en
dc.subject.other DNA sequence en
dc.subject.other drug sensitivity en
dc.subject.other Escherichia coli en
dc.subject.other gene cassette en
dc.subject.other heterozygote en
dc.subject.other hospital patient en
dc.subject.other human en
dc.subject.other integron en
dc.subject.other multidrug resistance en
dc.subject.other nonhuman en
dc.subject.other outpatient en
dc.subject.other phenotype en
dc.subject.other phylogenetic tree en
dc.subject.other polymerase chain reaction en
dc.subject.other poultry en
dc.subject.other prevalence en
dc.subject.other priority journal en
dc.subject.other pulsed field gel electrophoresis en
dc.subject.other reservoir en
dc.subject.other Animals en
dc.subject.other Blood en
dc.subject.other Disk Diffusion Antimicrobial Tests en
dc.subject.other DNA, Bacterial en
dc.subject.other Drug Resistance, Microbial en
dc.subject.other Drug Resistance, Multiple, Bacterial en
dc.subject.other Escherichia coli en
dc.subject.other Foodborne Diseases en
dc.subject.other Gastrointestinal Tract en
dc.subject.other Greece en
dc.subject.other Humans en
dc.subject.other Inpatients en
dc.subject.other Integrons en
dc.subject.other Outpatients en
dc.subject.other Phenotype en
dc.subject.other Polymerase Chain Reaction en
dc.subject.other Poultry en
dc.subject.other Sputum en
dc.subject.other Suppuration en
dc.subject.other Urine en
dc.subject.other Escherichia coli en
dc.title Prevalence and characterization of class 1 integrons in escherichia coli of poultry and human origin en
heal.type journalArticle en
heal.identifier.primary 10.1089/fpd.2009.0281 en
heal.identifier.secondary http://dx.doi.org/10.1089/fpd.2009.0281 en
heal.publicationDate 2009 en
heal.abstract A prospective study was conducted to determine the prevalence and the gene-cassette content of class 1 integrons in Escherichia coli of poultry and human origin. A total of 235 E. coli isolates were examined; 65 were derived from farm poultry, 80 from hospitalized, and 90 from nonhospitalized patients. Susceptibilities to a range of antimicrobial agents were determined by disk diffusion. Int1-specific polymerase chain reaction, conserved-segment polymerase chain reaction, and DNA sequencing were used to determine the presence, length, and content of integrons. The relatedness among the isolates was examined by pulsed-field gel electrophoresis of XbaI digests of genomic DNA. The integron carriage rate for poultry isolates was 49.2%, whereas the carriage rate for hospital isolates was 26.2% and for community 11.1%. Multidrug resistance (resistance to three or more classes of antibiotics) phenotypes were observed in 96.8% of the integron-positive isolates, whereas only 34.9% of nonintegron-carrying organisms were multidrug resistant (p<0.001). Seven integron types ranging in size from 663 to 2674bp were identified; six types were observed in poultry isolates, five in hospital, and three in community isolates. Each integron type carried a distinct gene-cassette combination. The most prevalent gene cassettes belonged to the aad and dfr families. Identical integrons were detected in E. coli of human and poultry origin. A large reservoir of integrons exists in E. coli of poultry origin. The horizontal transfer of class 1 integrons among bacteria of poultry and human origins may contribute in the dissemination of antimicrobial resistance. © 2009, Mary Ann Liebert, Inc. en
heal.journalName Foodborne Pathogens and Disease en
dc.identifier.doi 10.1089/fpd.2009.0281 en
dc.identifier.volume 6 en
dc.identifier.issue 10 en
dc.identifier.spage 1211 en
dc.identifier.epage 1218 en


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