Ultra-violet laser microbeam and optical trapping for cell micromanipulation

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dc.contributor.author Kotsifaki, D en
dc.contributor.author Makropoulou, M en
dc.contributor.author Serafetinides, AA en
dc.date.accessioned 2014-03-01T02:51:17Z
dc.date.available 2014-03-01T02:51:17Z
dc.date.issued 2007 en
dc.identifier.issn 16057422 en
dc.identifier.uri http://hdl.handle.net/123456789/35448
dc.subject He-Ne laser en
dc.subject Laser ablation en
dc.subject N2 laser en
dc.subject Optical tweezers en
dc.subject Yeast cells en
dc.subject.other Cells en
dc.subject.other Helium neon lasers en
dc.subject.other Laser ablation en
dc.subject.other Optical tweezers en
dc.subject.other Yeast en
dc.subject.other Laser microbeams en
dc.subject.other Microdissection en
dc.subject.other Micromanipulation en
dc.subject.other Optical trapping en
dc.subject.other Laser beams en
dc.title Ultra-violet laser microbeam and optical trapping for cell micromanipulation en
heal.type conferenceItem en
heal.identifier.primary 10.1117/12.741079 en
heal.identifier.secondary http://dx.doi.org/10.1117/12.741079 en
heal.identifier.secondary 65351O en
heal.publicationDate 2007 en
heal.abstract In recent years, lasers for optical trapping and micromanipulation of microscopic particles or cells and subcellular structures, both in vivo and in vitro, have gained remarkable interest in biomedical research and applications. On the other hand, a highly focused pulsed laser allows ablation and microdissection of biological material with high spatial resolution. In 1989 the microbeam field and the optical trapping field were merged by the first combination of laser microbeams and optical tweezers. Several laser sources are employed for the combination of a laser scalpel with an optical trapping device, under microscopic control. For example a pulsed laser microbeam that emits in the ultraviolet or the visible range of the electromagnetic spectrum can be used to form submicrometer cuts in biological material or to ablate parts specific cells. However, although the principles and the mechanisms of pulsed laser ablation have been well described for macroscopic interventions, the microbeam operation under microscopic guidance necessitates further investigation. In this work, we present the research and development efforts towards a pulsed UV microbeam laser system, the design and realization efforts towards a visible laser trapping system and the first results obtained on yeast cells by the combined system. The nitrogen laser microbeam setup, with special UV mirrors, lenses and a microscope in which ablation and the He-Ne optical tweezers are combined, performs the microsurgery and micromanipulation. This UV laser based setup provides good spatial resolution for microdissection. The beam quality delivered by the laser is of great importance in microscopy controlled ablating operations and therefore was extensively studied. en
heal.journalName Progress in Biomedical Optics and Imaging - Proceedings of SPIE en
dc.identifier.doi 10.1117/12.741079 en
dc.identifier.volume 6535 en

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