Atomic force microscopy quantitative and qualitative nanoscale characterization of collagen thin films

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dc.contributor.author Stylianou, A en
dc.contributor.author Yova, D en
dc.contributor.author Politopoulos, K en
dc.date.accessioned 2014-03-01T02:53:33Z
dc.date.available 2014-03-01T02:53:33Z
dc.date.issued 2012 en
dc.identifier.uri http://hdl.handle.net/123456789/36414
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-84856627531&partnerID=40&md5=b9bc6e58f87e5ca9eb2ea4e7de7b5ff9 en
dc.subject.other AFM en
dc.subject.other AFM imaging en
dc.subject.other Biological reaction en
dc.subject.other Biological samples en
dc.subject.other Cell response en
dc.subject.other Cellular process en
dc.subject.other Collagen fiber en
dc.subject.other Collagen fiber structure en
dc.subject.other Collagen surface en
dc.subject.other Culture substrate en
dc.subject.other Design and control en
dc.subject.other Different substrates en
dc.subject.other Extracellular matrices en
dc.subject.other Film formations en
dc.subject.other High-resolution imaging en
dc.subject.other Hydrodynamic flows en
dc.subject.other In-field en
dc.subject.other Nano scale en
dc.subject.other Nano-structured en
dc.subject.other Nanobiomaterials en
dc.subject.other Nanoscale characterization en
dc.subject.other Nanostructured Films en
dc.subject.other Nondestructive characterization en
dc.subject.other Polystyrene particle en
dc.subject.other Qualitative information en
dc.subject.other Second harmonics en
dc.subject.other Solution concentration en
dc.subject.other Surface characteristics en
dc.subject.other Surface characterization en
dc.subject.other Tissue regeneration en
dc.subject.other Atomic force microscopy en
dc.subject.other Biocompatibility en
dc.subject.other Cell culture en
dc.subject.other Medical applications en
dc.subject.other Mica en
dc.subject.other Nanostructures en
dc.subject.other Nondestructive examination en
dc.subject.other Optical properties en
dc.subject.other Polystyrenes en
dc.subject.other Scaffolds (biology) en
dc.subject.other Substrates en
dc.subject.other Surface reactions en
dc.subject.other Surface topography en
dc.subject.other Thin films en
dc.subject.other Tissue en
dc.subject.other Collagen en
dc.title Atomic force microscopy quantitative and qualitative nanoscale characterization of collagen thin films en
heal.type conferenceItem en
heal.publicationDate 2012 en
heal.abstract Collagen is the most abundant protein in mammals and is a basic component of the extracellular matrix. Due to its unique properties it is widely used as biomaterial, scaffold and culture substrate for cell and tissue regeneration studies. As the majority of the biological reactions occur on surfaces or interfaces and collagen fiber structure can trigger cell response it is of great importance to nanostructure collagen thin films. Collagen nanobiomaterials present improved surface characteristics and as a result they have wide applications in biomedicine, in fields where it is very critical to pre-determined the topography of the surfaces. Surface characterization in the nanoscale could be performed with Atomic Force Microscopy (AFM), which is a powerful tool and offers quantitative and qualitative information. Its ability of high resolution imaging and non destructive characterization under different conditions make it very attractive for biological samples investigation. The aim of this paper was to characterize the collagen fibers of the thin films formed on different substrates (glass, mica, polystyrene particle surfaces) and correlate their morphology with the characteristics of the used substrates, the formation methodologies (spin coating, hydrodynamic flow) and the original collagen solution. The clarification of the role that different parameters play in the formation of the films will enable the design and control of collagen based nanobiomaterials with pre-determined characteristics. The results demonstrated that AFM can be used to characterize nanostructured collagen thin films which, under appropriate control of a number of parameters (e.g. substrate, solution concentration, film formation procedure) it is possible to be formed with reproducible and pre-determined characteristics. These nanostructured films and the offered information by AFM imaging could be used in order to fully clarify the relationship between collagen surface nano-characteristics and cells behaviour. These films could be applied to direct cellular processes or cover non-biological surfaces, offering them biocompatibility, in a variety of medical applications. Additionally, these nanostructured patterns could model collagen rich tissues and facilitate the study their optical properties, like the generation of Second Harmonic. © 2012 Taylor & Francis Group, London. en
heal.journalName Emerging Technologies in Non-Destructive Testing V - Proceedings of the 5th Conference on Emerging Technologies in NDT en
dc.identifier.spage 415 en
dc.identifier.epage 420 en

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