Metabolic flux analysis as a tool for the elucidation of the metabolism of neurotransmitter glutamate

Chatziioannou, A; Palaiologos, G; Kolisis, FN

dc.contributor.author	Chatziioannou, A	en
dc.contributor.author	Palaiologos, G	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:19:17Z
dc.date.available	2014-03-01T01:19:17Z
dc.date.issued	2003	en
dc.identifier.issn	1096-7176	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15398
dc.subject	Aspartate Aminotransferase	en
dc.subject	Condition Number	en
dc.subject	Glutamate	en
dc.subject	Glutamate Release	en
dc.subject	Metabolic Flux Analysis	en
dc.subject	Potassium	en
dc.subject	Pyruvate Carboxylase	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	agents interacting with transmitter, hormone or drug receptors	en
dc.subject.other	glucose	en
dc.subject.other	glutamic acid	en
dc.subject.other	animal	en
dc.subject.other	article	en
dc.subject.other	biological model	en
dc.subject.other	brain	en
dc.subject.other	chemical model	en
dc.subject.other	comparative study	en
dc.subject.other	computer simulation	en
dc.subject.other	energy metabolism	en
dc.subject.other	evaluation	en
dc.subject.other	metabolism	en
dc.subject.other	nerve cell	en
dc.subject.other	physiology	en
dc.subject.other	rat	en
dc.subject.other	reproducibility	en
dc.subject.other	sensitivity and specificity	en
dc.subject.other	synaptic transmission	en
dc.subject.other	validation study	en
dc.subject.other	Animals	en
dc.subject.other	Brain	en
dc.subject.other	Computer Simulation	en
dc.subject.other	Energy Metabolism	en
dc.subject.other	Glucose	en
dc.subject.other	Glutamic Acid	en
dc.subject.other	Models, Chemical	en
dc.subject.other	Models, Neurological	en
dc.subject.other	Neurons	en
dc.subject.other	Neurotransmitter Agents	en
dc.subject.other	Rats	en
dc.subject.other	Reproducibility of Results	en
dc.subject.other	Sensitivity and Specificity	en
dc.subject.other	Synaptic Transmission	en
dc.title	Metabolic flux analysis as a tool for the elucidation of the metabolism of neurotransmitter glutamate	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1096-7176(03)00029-6	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1096-7176(03)00029-6	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	A flux analysis model for the metabolism of neurotransmitter glutamate is constructed, in order to study functional aspects of its metabolism. This work is based on the potassium [K+] evoked neurotransmitter glutamate released, as measured in a series of experiments of superfused rat or mouse brain preparations. These measurements are combined with data reported, concerning the metabolism of glutamate and its precursors, glutamine and glucose in rat cerebral cells in vivo. The proposed stoichiometry of the specific reaction network renders the model solvable. The classification procedure establishes that the measured fluxes are all balanceable and all non-measured fluxes can be calculated. The system is well posed with a condition number of 7.8536. The results emphasize the importance of phosphate activated glutaminase and aspartate aminotransferase in the metabolism of neurotransmitter glutamate. Reported data on the rate of the malate-aspartate shuttle, as well as the anaplerotic flux of the glial pyruvate carboxylase reaction are in agreement with the estimations calculated from the proposed model. (C) 2003 Elsevier Inc. All rights reserved.	en
heal.publisher	ACADEMIC PRESS INC ELSEVIER SCIENCE	en
heal.journalName	Metabolic Engineering	en
dc.identifier.doi	10.1016/S1096-7176(03)00029-6	en
dc.identifier.isi	ISI:000185462300006	en
dc.identifier.volume	5	en
dc.identifier.issue	3	en
dc.identifier.spage	201	en
dc.identifier.epage	210	en