STUDIES OF THE EFFECT OF ORGANIC-SOLVENTS ON THE STABILITY OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM

TSITSIMPIKOU, C; VOUTOU, D; CHRISTAKOPOULOS, P; KEKOS, D; MACRIS, BJ; KOLISIS, FN

dc.contributor.author	TSITSIMPIKOU, C	en
dc.contributor.author	VOUTOU, D	en
dc.contributor.author	CHRISTAKOPOULOS, P	en
dc.contributor.author	KEKOS, D	en
dc.contributor.author	MACRIS, BJ	en
dc.contributor.author	KOLISIS, FN	en
dc.date.accessioned	2014-03-01T01:42:56Z
dc.date.available	2014-03-01T01:42:56Z
dc.date.issued	1994	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/23996
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.title	STUDIES OF THE EFFECT OF ORGANIC-SOLVENTS ON THE STABILITY OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	The effect of organic solvents on the stability of beta-glucosidase in a powder form, isolated from Fusarium oxysporum, has been studied using several organic solvents of different degree of hydrophobicity. It was found that beta-glucosidase remains quite stable after a prolonged incubation in the presence of most of the organic solvents used, even at temperatures as high as 70 degrees C. Only dimethylformamide (DMF) and tetrahydrofuran (THF) reduce considerably the enzyme activity in a short preincubation period. Studies on the effect of the pH of the buffer used prior to lyophilization, as well as of exogenous added water to the incubation mixture, on enzyme stability show that it is more stable in pH 5.0 and in the lowest water content. In addition it was found that the presence of glucose in the lyophilization procedure gives a significant protection to the enzyme when it is incubated for 30 h in pentanol and n-hexane.	en
heal.publisher	CHAPMAN HALL LTD	en
heal.journalName	BIOTECHNOLOGY LETTERS	en
dc.identifier.isi	ISI:A1994MU96100011	en
dc.identifier.volume	16	en
dc.identifier.issue	1	en
dc.identifier.spage	57	en
dc.identifier.epage	62	en