Atomic force microscopy quantitative and qualitative nanoscale characterization of collagen thin films

Stylianou, A; Yova, D; Politopoulos, K

dc.contributor.author	Stylianou, A	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Politopoulos, K	en
dc.date.accessioned	2014-03-01T02:53:33Z
dc.date.available	2014-03-01T02:53:33Z
dc.date.issued	2012	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/36414
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-84856627531&partnerID=40&md5=b9bc6e58f87e5ca9eb2ea4e7de7b5ff9	en
dc.subject.other	AFM	en
dc.subject.other	AFM imaging	en
dc.subject.other	Biological reaction	en
dc.subject.other	Biological samples	en
dc.subject.other	Cell response	en
dc.subject.other	Cellular process	en
dc.subject.other	Collagen fiber	en
dc.subject.other	Collagen fiber structure	en
dc.subject.other	Collagen surface	en
dc.subject.other	Culture substrate	en
dc.subject.other	Design and control	en
dc.subject.other	Different substrates	en
dc.subject.other	Extracellular matrices	en
dc.subject.other	Film formations	en
dc.subject.other	High-resolution imaging	en
dc.subject.other	Hydrodynamic flows	en
dc.subject.other	In-field	en
dc.subject.other	Nano scale	en
dc.subject.other	Nano-structured	en
dc.subject.other	Nanobiomaterials	en
dc.subject.other	Nanoscale characterization	en
dc.subject.other	Nanostructured Films	en
dc.subject.other	Nondestructive characterization	en
dc.subject.other	Polystyrene particle	en
dc.subject.other	Qualitative information	en
dc.subject.other	Second harmonics	en
dc.subject.other	Solution concentration	en
dc.subject.other	Surface characteristics	en
dc.subject.other	Surface characterization	en
dc.subject.other	Tissue regeneration	en
dc.subject.other	Atomic force microscopy	en
dc.subject.other	Biocompatibility	en
dc.subject.other	Cell culture	en
dc.subject.other	Medical applications	en
dc.subject.other	Mica	en
dc.subject.other	Nanostructures	en
dc.subject.other	Nondestructive examination	en
dc.subject.other	Optical properties	en
dc.subject.other	Polystyrenes	en
dc.subject.other	Scaffolds (biology)	en
dc.subject.other	Substrates	en
dc.subject.other	Surface reactions	en
dc.subject.other	Surface topography	en
dc.subject.other	Thin films	en
dc.subject.other	Tissue	en
dc.subject.other	Collagen	en
dc.title	Atomic force microscopy quantitative and qualitative nanoscale characterization of collagen thin films	en
heal.type	conferenceItem	en
heal.publicationDate	2012	en
heal.abstract	Collagen is the most abundant protein in mammals and is a basic component of the extracellular matrix. Due to its unique properties it is widely used as biomaterial, scaffold and culture substrate for cell and tissue regeneration studies. As the majority of the biological reactions occur on surfaces or interfaces and collagen fiber structure can trigger cell response it is of great importance to nanostructure collagen thin films. Collagen nanobiomaterials present improved surface characteristics and as a result they have wide applications in biomedicine, in fields where it is very critical to pre-determined the topography of the surfaces. Surface characterization in the nanoscale could be performed with Atomic Force Microscopy (AFM), which is a powerful tool and offers quantitative and qualitative information. Its ability of high resolution imaging and non destructive characterization under different conditions make it very attractive for biological samples investigation. The aim of this paper was to characterize the collagen fibers of the thin films formed on different substrates (glass, mica, polystyrene particle surfaces) and correlate their morphology with the characteristics of the used substrates, the formation methodologies (spin coating, hydrodynamic flow) and the original collagen solution. The clarification of the role that different parameters play in the formation of the films will enable the design and control of collagen based nanobiomaterials with pre-determined characteristics. The results demonstrated that AFM can be used to characterize nanostructured collagen thin films which, under appropriate control of a number of parameters (e.g. substrate, solution concentration, film formation procedure) it is possible to be formed with reproducible and pre-determined characteristics. These nanostructured films and the offered information by AFM imaging could be used in order to fully clarify the relationship between collagen surface nano-characteristics and cells behaviour. These films could be applied to direct cellular processes or cover non-biological surfaces, offering them biocompatibility, in a variety of medical applications. Additionally, these nanostructured patterns could model collagen rich tissues and facilitate the study their optical properties, like the generation of Second Harmonic. © 2012 Taylor & Francis Group, London.	en
heal.journalName	Emerging Technologies in Non-Destructive Testing V - Proceedings of the 5th Conference on Emerging Technologies in NDT	en
dc.identifier.spage	415	en
dc.identifier.epage	420	en