Simultaneous production of glucose oxidase and catalase by Alternaria alternata

Caridis, K-A; Christakopoulos; Macris, BJ

dc.contributor.author	Caridis, K-A	en
dc.contributor.author	Christakopoulos	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:08:33Z
dc.date.available	2014-03-01T01:08:33Z
dc.date.issued	1991	en
dc.identifier.issn	0175-7598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/10558
dc.subject	Carbon Source	en
dc.subject	Catalase	en
dc.subject	Enzyme	en
dc.subject	Glucose Oxidase	en
dc.subject	Nitrogen	en
dc.subject	Number of Factors	en
dc.subject	Second Order Equation	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	catalase	en
dc.subject.other	glucose oxidase	en
dc.subject.other	alternaria	en
dc.subject.other	article	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	nonhuman	en
dc.subject.other	Alternaria	en
dc.subject.other	Alternaria alternata	en
dc.subject.other	Fungi	en
dc.title	Simultaneous production of glucose oxidase and catalase by Alternaria alternata	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/BF00169352	en
heal.identifier.secondary	http://dx.doi.org/10.1007/BF00169352	en
heal.language	English	en
heal.publicationDate	1991	en
heal.abstract	A number of factors affecting simultaneous production of cell-bound glucose oxidase and catalase by the fungus Alternaria alternata have been investigated. Consecutive optimization of the type and concentration of nitrogen and carbon source, the initial pH and growth temperature resulted in a simultaneous increase in glucose oxidase and catalase by 780% and 68% respectively. Two second-order equations, describing the combined effect of pH and temperature on the activity of each enzyme, revealed that glucose oxidase had its optima at pH 7.9 and 32.3-degrees-C and catalase at pH 8.5 and 18.1-degrees-C. Under certain growth conditions, yields as high as 23.5 and 18,100 units/g carbon source for glucose oxidase and catalase, respectively, were simultaneously obtained.	en
heal.publisher	SPRINGER VERLAG	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/BF00169352	en
dc.identifier.isi	ISI:A1991FD14300017	en
dc.identifier.volume	34	en
dc.identifier.issue	6	en
dc.identifier.spage	794	en
dc.identifier.epage	797	en