Correlating the effect of pretreatment on the enzymatic hydrolysis of straw

Koullas, DP; Christakopoulos, P; Kekos, D; Macris, BJ; Koukios, EG

dc.contributor.author	Koullas, DP	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Koukios, EG	en
dc.date.accessioned	2014-03-01T01:08:45Z
dc.date.available	2014-03-01T01:08:45Z
dc.date.issued	1992	en
dc.identifier.issn	0006-3592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/10663
dc.subject	ENZYMATIC HYDROLYSIS MODELS	en
dc.subject	PRETREATED LIGNOCELLULOSICS HYDROLYSIS	en
dc.subject	FUSARIUM-OXYSPORUM CELLULASES	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Cellulose - Hydrolysis	en
dc.subject.other	Enzymes	en
dc.subject.other	Lignin	en
dc.subject.other	Straw	en
dc.subject.other	Alkali-Treated Straw Cellulose	en
dc.subject.other	Avicell	en
dc.subject.other	Enzymatic Hydrolysis	en
dc.subject.other	Fusarium Oxysporum Strain F3	en
dc.subject.other	Biomass	en
dc.subject.other	cellulase	en
dc.subject.other	cellulose	en
dc.subject.other	hemicellulose	en
dc.subject.other	article	en
dc.subject.other	biodegradation	en
dc.subject.other	fusarium oxysporum	en
dc.subject.other	hydrolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	straw	en
dc.title	Correlating the effect of pretreatment on the enzymatic hydrolysis of straw	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/bit.260390116	en
heal.identifier.secondary	http://dx.doi.org/10.1002/bit.260390116	en
heal.language	English	en
heal.publicationDate	1992	en
heal.abstract	Avicell, Alkali-treated straw cellulose (ATSC), and wheat straw were ball-milled to reduce crystallinity; wheat straw was delignified by hot (120°C) sodium hydroxide solutions of various concentrations. The physically and chemically pretreated cellulosic materials were hydrolyzed by the cellulases of Fusarium oxysporum strain F3. Enzymic hydrolysis data were fitted by the hyperbolic correlation of Holtzapple, which involves two kinetic parameters, the maximum conversion (x(max)), and the enzymic hydrolysis time corresponding to 50% of x(max) (t( 1/2 )). An empirical correlation between x(max) and cellulose crystallinity, lignin content, and degree of delignification has been found under our experimental conditions. Complete cellulose hydrolysis is shown to be possible at less than 60% crystallinity indices or less than 10% lignin content.Avicell, Alkali-treated straw cellulose (ATSC), and wheat straw were ball-milled to reduce crystallinity; wheat straw was delignified by hot (120°C) sodium hydroxide solutions of various concentrations. The physically and chemically pretreated cellulosic materials were hydrolyzed by the cellulases of Fusarium oxysporum strain F3. Enzymic hydrolysis data were fitted by the hyperbolic correlation of Holtzapple, which involves two kinetic parameters, the maximum conversion (xmax), and the enzymic hydrolysis time corresponding to 50% of Xmax (t1/2). An empirical correlation between Xmax and cellulose crystallinity, lignin content, and degree of delignification has been found under our experimental conditions. Complete cellulose hydrolysis is shown to be possible at less than 60% crystallinity indices or less than 10% lignin content.	en
heal.publisher	JOHN WILEY & SONS INC	en
heal.journalName	Biotechnology and Bioengineering	en
dc.identifier.doi	10.1002/bit.260390116	en
dc.identifier.isi	ISI:A1992GW63400015	en
dc.identifier.volume	39	en
dc.identifier.issue	1	en
dc.identifier.spage	113	en
dc.identifier.epage	116	en