Production and characterization of extracellular lipase from Calvatia gigantea

Christakopoulos, P; Tzia, C; Kekos, D; Macris, BJ

dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Tzia, C	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:09:00Z
dc.date.available	2014-03-01T01:09:00Z
dc.date.issued	1992	en
dc.identifier.issn	0175-7598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/10795
dc.subject	Culture Medium	en
dc.subject	Enzyme	en
dc.subject	Kinetics	en
dc.subject	Nitrogen	en
dc.subject	Number of Factors	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	triacylglycerol lipase	en
dc.subject.other	article	en
dc.subject.other	biosynthesis	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	fungus	en
dc.title	Production and characterization of extracellular lipase from Calvatia gigantea	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/BF00174467	en
heal.identifier.secondary	http://dx.doi.org/10.1007/BF00174467	en
heal.language	English	en
heal.publicationDate	1992	en
heal.abstract	A number of factors affecting production of extracellular lipase by the edible fungus Calvatia gigantea were investigated. Consecutive optimization of carbon and nitrogen sources, initial pH of culture medium and growth temperature resulted in an increase in lipase activity of 87%. Under optimum conditions, activities as high as 22.4 units ml-1 of culture medium were obtained, competing favourably with most activities reported for other lipase hyperproducing microorganisms. The enzyme was optimally active at pH 7.0 and 300-degrees-C and had, at optimum pH, half-lives of 75.7 and 22.9 min at 45 and 55-degrees-C. Both high activity and kinetic characteristics of the enzyme make this process worthy of further investigation.	en
heal.publisher	SPRINGER VERLAG	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/BF00174467	en
dc.identifier.isi	ISI:A1992KB40600011	en
dc.identifier.volume	38	en
dc.identifier.issue	2	en
dc.identifier.spage	194	en
dc.identifier.epage	197	en