KINETIC-STUDY OF LIPASE-CATALYZED ESTERIFICATION REACTIONS IN WATER-IN-OIL MICROEMULSIONS

STAMATIS, H; XENAKIS, A; MENGE, U; KOLISIS, FN

dc.contributor.author	STAMATIS, H	en
dc.contributor.author	XENAKIS, A	en
dc.contributor.author	MENGE, U	en
dc.contributor.author	KOLISIS, FN	en
dc.date.accessioned	2014-03-01T01:09:26Z
dc.date.available	2014-03-01T01:09:26Z
dc.date.issued	1993	en
dc.identifier.issn	0006-3592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/10980
dc.subject	LIPASE	en
dc.subject	MICROEMULSIONS	en
dc.subject	REVERSE MICELLES	en
dc.subject	ESTERIFICATION	en
dc.subject	KINETICS	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	REVERSE MICELLES	en
dc.subject.other	ORGANIC-SOLVENTS	en
dc.subject.other	FATTY-ACIDS	en
dc.subject.other	ENZYMES	en
dc.subject.other	SURFACTANTS	en
dc.subject.other	ENZYMOLOGY	en
dc.subject.other	SYSTEMS	en
dc.subject.other	STATE	en
dc.title	KINETIC-STUDY OF LIPASE-CATALYZED ESTERIFICATION REACTIONS IN WATER-IN-OIL MICROEMULSIONS	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/bit.260420803	en
heal.identifier.secondary	http://dx.doi.org/10.1002/bit.260420803	en
heal.language	English	en
heal.publicationDate	1993	en
heal.abstract	The kinetics of the esterification of lauric acid by (-)menthol, catalyzed by Penicillium simplicissimum lipase, was studied in water/bis-(2-ethylhexyl)sulfosuccinate sodium salt (AOT)/isooctane microemulsions. Due to their low water content, microemulsions assist in reversing the direction of lipase activity, favoring synthetic reactions. The kinetics of this synthesis follows a Ping-Pong Bi-Bi mechanism. The values of all apparent kinetic parameters were determined. The theoretical model for the expression of enzymic activity in reverse micelles, proposed by Verhaert et al. (Verhaert, R., Hilhorst, R., Vermue, M., Schaafsma, T.J., Veeger, C. 1990. Eur. J. Biochem. 187: 59-72) was extended to express the lipase activity in an esterification reaction involving two hydrophobic substrates in microemulsion systems. The model takes into account the partitioning of the substrates between the various phases and allows the calculation of the intrinsic kinetic constants. The experimental results showing the dependence of the initial velocity on the hydration ratio, w(o) = [H2O]/[AOT], of the reverse micelles, were in accordance with the theoretically predicted pattern. (C) 1993 John Wiley & Sons, Inc.	en
heal.publisher	JOHN WILEY & SONS INC	en
heal.journalName	BIOTECHNOLOGY AND BIOENGINEERING	en
dc.identifier.doi	10.1002/bit.260420803	en
dc.identifier.isi	ISI:A1993LY46900002	en
dc.identifier.volume	42	en
dc.identifier.issue	8	en
dc.identifier.spage	931	en
dc.identifier.epage	937	en