Enzymatic synthesis of butyryl-rutin ester in organic solvents and its cytogenetic effects in mammalian cells in culture

Kodelia, G; Athanasiou, K; Kolisis, FN

dc.contributor.author	Kodelia, G	en
dc.contributor.author	Athanasiou, K	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:09:52Z
dc.date.available	2014-03-01T01:09:52Z
dc.date.issued	1994	en
dc.identifier.issn	0273-2289	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11221
dc.subject	cytogenetic effects	en
dc.subject	Enzymic catalysis in organic solvents	en
dc.subject	flavonoids	en
dc.subject	micronuclei	en
dc.subject	rutin	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	FLAVONOIDS	en
dc.subject.other	INVITRO	en
dc.subject.other	MONOGLYCOSIDES	en
dc.subject.other	MUTAGENICITY	en
dc.subject.other	KAEMPFEROL	en
dc.subject.other	QUERCETIN	en
dc.subject.other	CATALYSIS	en
dc.title	Enzymatic synthesis of butyryl-rutin ester in organic solvents and its cytogenetic effects in mammalian cells in culture	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/BF02779657	en
heal.identifier.secondary	http://dx.doi.org/10.1007/BF02779657	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	Enzymic acylation of a flavonoid, rutin, with trichloroethylbutyrate (TCEB) has been performed by subtilisin protease in anhydrous pyridine solution. The addition of a hydrophobic compound on rutin is expected to change the hydrophilic/hydrophobic balance of the molecule, giving new properties to this compound. This work aimed at investigating the various cytological properties of the rutin-ester and compared them with those of the native molecule. No difference in the levels of sister chromosomes exchange (SCE) between rutin and rutin-ester treated cells at doses varying from 25 to 200 μg/mL was found. On the contrary impressive difference in the induced frequency of micronuclei (MN) between rutin and rutin ester treated cells was observed, for example, at a dose of 100 μg/mL of rutin were 3.5% MN counted, whereas for a similar dose treatment with rutin-ester a frequency of 8% of MN was found. The fact that rutin-ester is causing significantly higher levels of MN than the rutin alone can be considered as a manifestation of a higher action of the agent on the chromosome owing to its easier penetration in to the cell after its esterification. © 1994 Humana Press Inc.	en
heal.publisher	Humana Press	en
heal.journalName	Applied Biochemistry and Biotechnology	en
dc.identifier.doi	10.1007/BF02779657	en
dc.identifier.isi	ISI:A1994NC37300001	en
dc.identifier.volume	44	en
dc.identifier.issue	3	en
dc.identifier.spage	205	en
dc.identifier.epage	212	en