LIPASE OF PSEUDOMONAS-CEPACIA FOR BIOTECHNOLOGICAL PURPOSES - PURIFICATION, CRYSTALLIZATION AND CHARACTERIZATION

BORNSCHEUER, U; REIF, OW; LAUSCH, R; FREITAG, R; SCHEPER, T; KOLISIS, FN; MENGE, U

dc.contributor.author	BORNSCHEUER, U	en
dc.contributor.author	REIF, OW	en
dc.contributor.author	LAUSCH, R	en
dc.contributor.author	FREITAG, R	en
dc.contributor.author	SCHEPER, T	en
dc.contributor.author	KOLISIS, FN	en
dc.contributor.author	MENGE, U	en
dc.date.accessioned	2014-03-01T01:09:58Z
dc.date.available	2014-03-01T01:09:58Z
dc.date.issued	1994	en
dc.identifier.issn	0304-4165	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11267
dc.subject	TRIACYLGLYCEROL LIPASE	en
dc.subject	PROTEIN PURIFICATION	en
dc.subject	(P-CEPACIA)	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biophysics	en
dc.subject.other	FLUORESCENS	en
dc.subject.other	PROTEINS	en
dc.subject.other	CLONING	en
dc.subject.other	GENE	en
dc.title	LIPASE OF PSEUDOMONAS-CEPACIA FOR BIOTECHNOLOGICAL PURPOSES - PURIFICATION, CRYSTALLIZATION AND CHARACTERIZATION	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/0304-4165(94)90151-1	en
heal.identifier.secondary	http://dx.doi.org/10.1016/0304-4165(94)90151-1	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	Commercial lipase (triacylglycerol lipase, EC 3.1.1.3) of Pseudomonas cepacia (Amano) has been purified to homogeneity by a single chromatography on phenyl Sepharose. The eluted lipase crystallized spontaneously at 4 degrees C in the eluent, containing 58-69% 2-propanol. The yield of the lipase was 87-100% and the specific activity during the hydrolysis of triolein 5800 U/mg protein. This protein has a molecular weight of 34.1 kDa as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Its purity was determined by SDS-PAGE and capillary zone electrophoresis to be greater than or equal to 99%. Immobilization on Sepharose increased its stability in organic solvents. This lipase of P. cepacia differs from that of other Pseudomonas strains in respect to substrate specificity and during crystallization. It exhibits a high stability in organic solvents and supercritical carbon dioxide.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS	en
dc.identifier.doi	10.1016/0304-4165(94)90151-1	en
dc.identifier.isi	ISI:A1994PJ53600010	en
dc.identifier.volume	1201	en
dc.identifier.issue	1	en
dc.identifier.spage	55	en
dc.identifier.epage	60	en