OPTIMIZATION OF BETA-GLUCOSIDASE CATALYZED SYNTHESIS OF TRISACCHARIDES FROM CELLOBIOSE AND GENTIOBIOSE

CHRISTAKOPOULOS, P; KEKOS, D; MACRIS, BJ; GOODENOUGH, PW; BHAT, MK

dc.contributor.author	CHRISTAKOPOULOS, P	en
dc.contributor.author	KEKOS, D	en
dc.contributor.author	MACRIS, BJ	en
dc.contributor.author	GOODENOUGH, PW	en
dc.contributor.author	BHAT, MK	en
dc.date.accessioned	2014-03-01T01:10:04Z
dc.date.available	2014-03-01T01:10:04Z
dc.date.issued	1994	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11303
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	OLIGOSACCHARIDE SYNTHESIS	en
dc.subject.other	PURIFICATION	en
dc.title	OPTIMIZATION OF BETA-GLUCOSIDASE CATALYZED SYNTHESIS OF TRISACCHARIDES FROM CELLOBIOSE AND GENTIOBIOSE	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/BF00128605	en
heal.identifier.secondary	http://dx.doi.org/10.1007/BF00128605	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	Purified beta-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15-20 % of the initial substrate concentration. These results suggested that beta-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.	en
heal.publisher	CHAPMAN HALL LTD	en
heal.journalName	BIOTECHNOLOGY LETTERS	en
dc.identifier.doi	10.1007/BF00128605	en
dc.identifier.isi	ISI:A1994NW11900008	en
dc.identifier.volume	16	en
dc.identifier.issue	6	en
dc.identifier.spage	587	en
dc.identifier.epage	592	en