Factors regulating production of glucose oxidase by Aspergillus niger

Hatzinikolaou, DG; Macris, BJ

dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:11:01Z
dc.date.available	2014-03-01T01:11:01Z
dc.date.issued	1995	en
dc.identifier.issn	0141-0229	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11528
dc.subject	Aspergillus niger	en
dc.subject	Glucose oxidase	en
dc.subject	Optimization	en
dc.subject	Production	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Calcium carbide	en
dc.subject.other	Calcium compounds	en
dc.subject.other	Carbonates	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Fungi	en
dc.subject.other	Molasses	en
dc.subject.other	pH	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Sterilization (cleaning)	en
dc.subject.other	Sugar (sucrose)	en
dc.subject.other	Enzyme activity	en
dc.subject.other	Enzymes	en
dc.subject.other	calcium carbonate	en
dc.subject.other	glucose oxidase	en
dc.subject.other	nitrogen	en
dc.subject.other	article	en
dc.subject.other	aspergillus niger	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme assay	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	glucose metabolism	en
dc.subject.other	nonhuman	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Fungi	en
dc.title	Factors regulating production of glucose oxidase by Aspergillus niger	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/0141-0229(95)91708-7	en
heal.identifier.secondary	http://dx.doi.org/10.1016/0141-0229(95)91708-7	en
heal.language	English	en
heal.publicationDate	1995	en
heal.abstract	Certain factors affecting production of extracellular and cell-bound glucose oxidase by Aspergillus niger were investigated. The intention was to maximize total glucose oxidase activity of academic and potential commercial application by the selection of the appropriate strain and consecutive optimization of growth media and conditions. It war; possible to identify combinations resulting in the utilization of molasses as the best carbon source and enhancing enzyme activity approximately 40-fold. Glucose oxidase activities as high as 5.7Uml-1 were produced, comparing favorably with those reported for other enzyme-producing microorganisms. These activity levels were obtained with molasses, indicating an economically attractive process for enzyme production. In addition, our work identified CaCO3 as a particularly strong inducer of glucose oxidase activity.Certain factors affecting production of extracellular and cell-bound glucose oxidase by Aspergillus niger were investigated. The intention was to maximize total glucose oxidase activity of academic and potential commercial application by the selection of the appropriate strain and consecutive optimization of growth media and conditions. It was possible to identify combinations resulting in the utilization of molasses as the best carbon source and enhancing enzyme activity approximately 40-fold. Glucose oxidase activities as high as 5.7 U ml-1 were produced, comparing favorably with those reported for other enzyme-producing microorganisms. These activity levels were obtained with molasses, indicating an economically attractive process for enzyme production. In addition, our work identified CaCO3 as a particularly strong inducer of glucose oxidase activity.	en
heal.publisher	Elsevier Science Publ Co Inc, New York, NY, United States	en
heal.journalName	Enzyme and Microbial Technology	en
dc.identifier.doi	10.1016/0141-0229(95)91708-7	en
dc.identifier.isi	ISI:A1995RA81600008	en
dc.identifier.volume	17	en
dc.identifier.issue	6	en
dc.identifier.spage	530	en
dc.identifier.epage	534	en