A new glucose oxidase from Aspergillus niger: Characterization and regulation studies of enzyme and gene

Hatzinikolaou, DG; Hansen, OC; Macris, BJ; Tingey, A; Kekos, D; Goodenough, P; Stougaard, P

dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Hansen, OC	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Tingey, A	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Goodenough, P	en
dc.contributor.author	Stougaard, P	en
dc.date.accessioned	2014-03-01T01:11:36Z
dc.date.available	2014-03-01T01:11:36Z
dc.date.issued	1996	en
dc.identifier.issn	0175-7598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11735
dc.subject	Aspergillus Niger	en
dc.subject	Calcium Carbonate	en
dc.subject	Dna Sequence Analysis	en
dc.subject	Enzyme	en
dc.subject	Glucose Oxidase	en
dc.subject	Kinetics	en
dc.subject	Open Reading Frame	en
dc.subject	Pentose Phosphate Pathway	en
dc.subject	glucose 6 phosphate	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	article	en
dc.subject.other	aspergillus niger	en
dc.subject.other	controlled study	en
dc.subject.other	dna sequence	en
dc.subject.other	enzyme analysis	en
dc.subject.other	enzyme isolation	en
dc.subject.other	gene expression regulation	en
dc.subject.other	glycolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	pentose phosphate cycle	en
dc.subject.other	transcription regulation	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Base Sequence	en
dc.subject.other	Calcium Carbonate	en
dc.subject.other	Gene Expression Regulation, Fungal	en
dc.subject.other	Genes, Fungal	en
dc.subject.other	Gluconates	en
dc.subject.other	Glucose Oxidase	en
dc.subject.other	Heat	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Kinetics	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Promoter Regions (Genetics)	en
dc.subject.other	RNA, Fungal	en
dc.subject.other	RNA, Messenger	en
dc.subject.other	Sequence Analysis, DNA	en
dc.subject.other	Sequence Homology, Nucleic Acid	en
dc.subject.other	Transcription, Genetic	en
dc.subject.other	Aspergillus niger	en
dc.title	A new glucose oxidase from Aspergillus niger: Characterization and regulation studies of enzyme and gene	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s002530050832	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s002530050832	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	A new glucose oxidase from Aspergillus niger was isolated and characterized. The enzyme showed different kinetic and stability characteristics when compared to a commercially available batch of A. niger glucose oxidase. The gene encoding the new glucose oxidase was isolated and DNA sequence analysis of the coding region showed 80% identity to the sequence of a glucose oxidase gene previously published. However, the similarity of the non-coding sequences up- and downstream of the open reading frame was much less, showing only 66% and 50% identity respectively. Despite the low degree of similarity between the promotor region of the new gene and the previously published one, the new glucose oxidase was likewise induced by calcium carbonate. In addition, we showed that this induction occurred on the transcriptional level. Observations concerning the effect of gluconolactone and the levels of glucose-6-phosphate isomerase upon calcium carbonate induction suggested that the enhancement of glucose oxidase biosynthesis by calcium carbonate was accompanied by a metabolic shift from glycolysis to the pentose phosphate pathway.	en
heal.publisher	SPRINGER VERLAG	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s002530050832	en
dc.identifier.isi	ISI:A1996VW43200009	en
dc.identifier.volume	46	en
dc.identifier.issue	4	en
dc.identifier.spage	371	en
dc.identifier.epage	381	en