Cross-synergism in enzymatic hydrolysis of lignocellulosics: Mathematical correlations according to a hyperbolic model

Tarantili, PA; Koullas, DP; Christakopoulos, P; Kekos, D; Koukios, EG; Macris, BJ

dc.contributor.author	Tarantili, PA	en
dc.contributor.author	Koullas, DP	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Koukios, EG	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:11:50Z
dc.date.available	2014-03-01T01:11:50Z
dc.date.issued	1996	en
dc.identifier.issn	0961-9534	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/11826
dc.subject	Cross-synergism in enzymatic hydrolysis	en
dc.subject	enzymatic hydrolysis models	en
dc.subject	Fusarium oxysporum	en
dc.subject	Neurospora crassa cellulases	en
dc.subject	pretreated lignocellulosics hydrolysis	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Cellulose	en
dc.subject.other	Chemical reactions	en
dc.subject.other	Correlation methods	en
dc.subject.other	Energy conversion	en
dc.subject.other	Enzymes	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Mathematical models	en
dc.subject.other	Microorganisms	en
dc.subject.other	Mixtures	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Straw	en
dc.subject.other	Substrates	en
dc.subject.other	Alkali treated straw cellulose	en
dc.subject.other	Avicell	en
dc.subject.other	Cross synergism	en
dc.subject.other	Crystallinity index	en
dc.subject.other	Delignification	en
dc.subject.other	Enzymatic hydrolysis	en
dc.subject.other	Filter paper	en
dc.subject.other	Hyperbolic model	en
dc.subject.other	Lignocellulosics	en
dc.subject.other	Maximum conversion	en
dc.subject.other	Biomass	en
dc.title	Cross-synergism in enzymatic hydrolysis of lignocellulosics: Mathematical correlations according to a hyperbolic model	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/0961-9534(95)00059-3	en
heal.identifier.secondary	http://dx.doi.org/10.1016/0961-9534(95)00059-3	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	The effect of cross-synergism in enzymatic hydrolysis of ball-milled Avicell, alkali-treated straw cellulose (ATSC), cotton and filter paper was investigated using mixtures of Fusarium oxysporum and Neurospora crassa enzymes. The experimental data were fitted according to an empirical hyperbolic model which utilized two parameters, the maximum conversion (x(max)) and the enzymatic hydrolysis time corresponding to 50% of x(max) (1/2). The model can predict conversion of polysaccharides as a function of hydrolysis time. Both model parameters were found to be strongly dependent on the crystallinity index as well as on the degree of delignification of the substrate. Up to 60% cellulose hydrolysis can be achieved when the crystallinity index of Avicell is reduced from 94.8% to 63.3%. The percentage increase of x(max) due to delignification was higher than the corresponding increase of t(1/2). The extent of cross-synergism depends strongly on crystallinity index and degree of delignification. This type of synergism has been found to be significant in the case of substrates which are resistant to hydrolysis, such as Avicell (with high crystallinity index) or cotton. Cross-synergistic phenomena caused by enzymatic mixtures can double cellulose hydrolysis yield with delignified straw as compared to the hydrolysis yields achieved by single-microorganism cellulases.The effect of cross-synergism in enzymatic hydrolysis of ball-milled Avicell, alkali-treated straw cellulose (ATSC), cotton and filter paper was investigated using mixtures of Fusarium oxysporum and Neurospora crassa enzymes. The experimental data were fitted according to an empirical hyperbolic model which utilized two parameters, the maximum conversion (xmax) and the enzymatic hydrolysis time corresponding to 50% of xmax (t1/2). The model can predict conversion of polysaccharides as a function of hydrolysis time. Both model parameters were found to be strongly dependent on the crystallinity index as well as on the degree of delignification of the substrate. Up to 60% cellulose hydrolysis can be achieved when the crystallinity index of Avicell is reduced from 94.8% to 63.3%. The percentage increase of xmax due to delignification was higher than the corresponding increase of t1/2. The extent of cross-synergism depends strongly on crystallinity index and degree of delignification. This type of synergism has been found to be significant in the case of substrates which are resistant to hydrolysis, such as Avicell (with high crystallinity index) or cotton. Cross-synergistic phenomena caused by enzymatic mixtures can double cellulose hydrolysis yield with delignified straw as compared to the hydrolysis yields achieved by single-microorganism cellulases.	en
heal.publisher	Pergamon Press Inc, Tarrytown, NY, United States	en
heal.journalName	Biomass and Bioenergy	en
dc.identifier.doi	10.1016/0961-9534(95)00059-3	en
dc.identifier.isi	ISI:A1996UQ43100003	en
dc.identifier.volume	10	en
dc.identifier.issue	4	en
dc.identifier.spage	213	en
dc.identifier.epage	219	en