Production and partial characterization of xylanase from Fusarium oxysporum

Christakopoulos, P; Mamma, D; Nerinckx, W; Kekos, D; Macris, B; Claeyssens, M

dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Mamma, D	en
dc.contributor.author	Nerinckx, W	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, B	en
dc.contributor.author	Claeyssens, M	en
dc.date.accessioned	2014-03-01T01:12:12Z
dc.date.available	2014-03-01T01:12:12Z
dc.date.issued	1996	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12006
dc.subject	Chromogenic substrates	en
dc.subject	Fusarium oxysporum	en
dc.subject	pH stability	en
dc.subject	Xylanase production	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Carbon	en
dc.subject.other	Cell culture	en
dc.subject.other	Fungi	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Nitrogen	en
dc.subject.other	pH effects	en
dc.subject.other	Substrates	en
dc.subject.other	Thermal effects	en
dc.subject.other	Xylose	en
dc.subject.other	Chromogenic substrates	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Methylumbelliferyl glycosides	en
dc.subject.other	Xylanase	en
dc.subject.other	Xylobiose	en
dc.subject.other	Xylosidase	en
dc.subject.other	Enzymes	en
dc.subject.other	carbon	en
dc.subject.other	nitrogen	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	xylose	en
dc.subject.other	article	en
dc.subject.other	culture medium	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	fungus growth	en
dc.subject.other	fusarium oxysporum	en
dc.subject.other	isoelectric focusing	en
dc.subject.other	ph	en
dc.subject.other	priority journal	en
dc.subject.other	temperature	en
dc.subject.other	time	en
dc.title	Production and partial characterization of xylanase from Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0960-8524(96)00091-0	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0960-8524(96)00091-0	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively.Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major, one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Bioresource Technology	en
dc.identifier.doi	10.1016/S0960-8524(96)00091-0	en
dc.identifier.isi	ISI:A1996WG39800004	en
dc.identifier.volume	58	en
dc.identifier.issue	2	en
dc.identifier.spage	115	en
dc.identifier.epage	119	en