Structural studies of lecithin- and AOT-based water-in-oil microemulsions, in the presence of lipase

Avramiotis, S; Stamatis, H; Kolisis, FN; Lianos, P; Xenakis, A

dc.contributor.author	Avramiotis, S	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Kolisis, FN	en
dc.contributor.author	Lianos, P	en
dc.contributor.author	Xenakis, A	en
dc.date.accessioned	2014-03-01T01:12:19Z
dc.date.available	2014-03-01T01:12:19Z
dc.date.issued	1996	en
dc.identifier.issn	0743-7463	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12060
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	FLUORESCENCE ENERGY-TRANSFER	en
dc.subject.other	PSEUDOMONAS-CEPACIA LIPASE	en
dc.subject.other	REVERSE MICELLES	en
dc.subject.other	ESTERIFICATION REACTIONS	en
dc.subject.other	W/O MICROEMULSIONS	en
dc.subject.other	LOCALIZATION	en
dc.subject.other	BEHAVIOR	en
dc.title	Structural studies of lecithin- and AOT-based water-in-oil microemulsions, in the presence of lipase	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/la9606862	en
heal.identifier.secondary	http://dx.doi.org/10.1021/la9606862	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	Water-in-oil microemulsions based on lecithin or AOT were used to solubilized lipase from Pseudomonas cepacia. The enzyme activity was tested in the catalysis of an esterification reaction between lauric acid and various short chained aliphatic alcohols. Lipase was found to be more specific for propanol in the lecithin-based system, while in the AOT microemulsions the enzyme did not show any alcohol preference. Fluorescence energy transfer measurements were applied to detect the site of localization of the enzyme molecule within the different domains of the microemulsion droplets. In both cases the enzyme was found to be close to the surfactant interface, with the efficiency of the transfer being more pronounced in the AOT systems. Time-resolved luminescence quenching was also applied to both systems, to probe structural modifications of the microemulsions induced by the presence of lipase. Communication between the dispersed aqueous microdomains, as expressed by the observation of percolative phenomena, was found to be affected.	en
heal.publisher	AMER CHEMICAL SOC	en
heal.journalName	Langmuir	en
dc.identifier.doi	10.1021/la9606862	en
dc.identifier.isi	ISI:A1996WA87700027	en
dc.identifier.volume	12	en
dc.identifier.issue	26	en
dc.identifier.spage	6320	en
dc.identifier.epage	6325	en