Comparative studies on the sugar esters synthesis catalysed by Candida antarctica and Candida rugosa lipases in hexane

Tsitsimpikou, C; Daflos, H; Kolisis, FN

dc.contributor.author	Tsitsimpikou, C	en
dc.contributor.author	Daflos, H	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:12:41Z
dc.date.available	2014-03-01T01:12:41Z
dc.date.issued	1997	en
dc.identifier.issn	1381-1177	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12204
dc.subject	Lipase	en
dc.subject	Sugar-fatty acid esters	en
dc.subject	Water activity	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	Acylation	en
dc.subject.other	Bacteria	en
dc.subject.other	Enzyme immobilization	en
dc.subject.other	Esters	en
dc.subject.other	Fructose	en
dc.subject.other	Glucose	en
dc.subject.other	Organic solvents	en
dc.subject.other	Paraffins	en
dc.subject.other	Sugars	en
dc.subject.other	Lipases	en
dc.subject.other	Monosaccharides	en
dc.subject.other	Enzymes	en
dc.subject.other	arabinose	en
dc.subject.other	fructose	en
dc.subject.other	glucose	en
dc.subject.other	mannose	en
dc.subject.other	monosaccharide	en
dc.subject.other	triacylglycerol lipase	en
dc.subject.other	acylation	en
dc.subject.other	article	en
dc.subject.other	chemical structure	en
dc.subject.other	enzyme activity	en
dc.subject.other	nonhuman	en
dc.subject.other	structure analysis	en
dc.subject.other	temperature	en
dc.title	Comparative studies on the sugar esters synthesis catalysed by Candida antarctica and Candida rugosa lipases in hexane	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1381-1177(97)89617-8	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1381-1177(97)89617-8	en
heal.language	English	en
heal.publicationDate	1997	en
heal.abstract	The regioselective acylation of monosaccharides, such as glucose, fructose, mannose and arabinose with lauric acid (C12) has been investigated in hexane using commercial Candida rugosa lipase (EC 3.1.1.3) and Candida antarctica (NOVO-ZYME) lipase immobilised on anionic resin. In order to overcome sugars insolubility in hexane, sugars were introduced in the reaction mixture preabsorbed on silica gel. The chemical structure of the products has been determined by 1H NMR analysis. The two enzymes tested seemed to prefer hexoses rather than pentoses as the sugar substrate. They exhibited different water activity optimums as well as different optimum sugar/acyl donor molar ratios. Water activity control throughout the reaction and temperature increase from 30 to 50°C enhanced sugar ester production using both lipases.The regioselective acylation of monosaccharides, such as glucose, fructose, mannose and arabinose with lauric acid (C12) has been investigated in hexane using commercial Candida rugosa lipase (EC 3.1.1.3) and Candida antarctica (NOVOZYME) lipase immobilised on anionic resin. In order to overcome sugars insolubility in hexane, sugars were introduced in the reaction mixture preabsorbed on silica gel. The chemical structure of the products has been determined by H NMR analysis. The two enzymes tested seemed to prefer hexoses rather than pentoses as the sugar substrate. They exhibited different water activity optimums as well as different optimum sugar/acyl donor molar ratios. Water activity control throughout the reaction and temperature increase from 30 to 50°C enhanced sugar ester production using both lipases.	en
heal.publisher	Elsevier Sci B.V., Amsterdam, Netherlands	en
heal.journalName	Journal of Molecular Catalysis - B Enzymatic	en
dc.identifier.doi	10.1016/S1381-1177(97)89617-8	en
dc.identifier.isi	ISI:A1997XF11500024	en
dc.identifier.volume	3	en
dc.identifier.issue	1-4	en
dc.identifier.spage	189	en
dc.identifier.epage	192	en