Role of methanol on the catalytic behavior of β-glucosidase from Fusarium oxysporum

Tsitsimpikou, C; Christakopoulos, P; Makropoulou, M; Kekos, D; Macris, BJ; Kolisis, FN

dc.contributor.author	Tsitsimpikou, C	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:13:19Z
dc.date.available	2014-03-01T01:13:19Z
dc.date.issued	1997	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12426
dc.subject	fusarium oxysporum	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	beta glucosidase	en
dc.subject.other	cellobiose	en
dc.subject.other	glucose	en
dc.subject.other	methanol	en
dc.subject.other	article	en
dc.subject.other	catalysis	en
dc.subject.other	fusarium oxysporum	en
dc.subject.other	glycosylation	en
dc.subject.other	hydrolysis	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Role of methanol on the catalytic behavior of β-glucosidase from Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1018306802099	en
heal.identifier.secondary	http://dx.doi.org/10.1023/A:1018306802099	en
heal.language	English	en
heal.publicationDate	1997	en
heal.abstract	Fusarium oxysporum β-glucosidase catalyses the transglycosylation and reverse hydrolysis reaction in an aqueous solution, in the presence of a second nucleophile stronger than water, such as methanol. In transglycosylation, the K(m) values of cellobiose for the trans reaction and hydrolysis were 138.2 mM and 356.5 mM respectively and the competitive role of methanol was shown. The K(m) value of methanol was calculated to be 1.41 M. In the reverse hydrolysis the influence of the donor concentration, up to 90% w/v glucose, was investigated and the competitive role of methanol as a nucleophilic acceptor of the glycosyl moiety was also demonstrated.	en
heal.publisher	CHAPMAN HALL LTD	en
heal.journalName	Biotechnology Letters	en
dc.identifier.doi	10.1023/A:1018306802099	en
dc.identifier.isi	ISI:A1997WJ00400008	en
dc.identifier.volume	19	en
dc.identifier.issue	1	en
dc.identifier.spage	31	en
dc.identifier.epage	33	en