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Dielectric study of the double helix to single coil transition of DNA

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dc.contributor.author Georgakilas, AG en
dc.contributor.author Haveles, KS en
dc.contributor.author Sideris, EG en
dc.contributor.author Konsta, AA en
dc.date.accessioned 2014-03-01T01:13:41Z
dc.date.available 2014-03-01T01:13:41Z
dc.date.issued 1998 en
dc.identifier.issn 1070-9878 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/12657
dc.subject Aqueous Solution en
dc.subject Dielectric Relaxation en
dc.subject Hydrogen Bond en
dc.subject Nitrogen en
dc.subject.classification Engineering, Electrical & Electronic en
dc.subject.classification Physics, Applied en
dc.subject.other STRAND BREAK FORMATION en
dc.subject.other AQUEOUS-SOLUTION en
dc.subject.other CONDUCTIVITY en
dc.subject.other TEMPERATURE en
dc.subject.other FLUCTUATION en
dc.subject.other DISPERSION en
dc.subject.other RELAXATION en
dc.subject.other SYSTEMS en
dc.title Dielectric study of the double helix to single coil transition of DNA en
heal.type journalArticle en
heal.identifier.primary 10.1109/94.660755 en
heal.identifier.secondary http://dx.doi.org/10.1109/94.660755 en
heal.language English en
heal.publicationDate 1998 en
heal.abstract In this work, we investigate the dielectric behavior of mammalian DNA macromolecules of different sizes in aqueous solution, by dielectric relaxation spectroscopy in the frequency range 100 Hz to 1 MHz, during the double helix to single coil thermal transition, in association with the gradual disruption of the hydrogen bonds between the complementary nitrogenous bases of the DNA double helix, measured by thermal transition spectrophotometry. Our results have shown that, when the temperature of DNA samples is gradually increasing, changes in specific conductivity σdc precede changes in A260 absorbance at the UV region, where A260 is related to the disruption of the hydrogen bonds between the two DNA strands. The presented experimental evidence extends the validation of multistage models for this transition phase to the case of large size mammalian native macromolecular DNA. © 1998 IEEE. en
heal.publisher IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC en
heal.journalName IEEE Transactions on Dielectrics and Electrical Insulation en
dc.identifier.doi 10.1109/94.660755 en
dc.identifier.isi ISI:000072527500006 en
dc.identifier.volume 5 en
dc.identifier.issue 1 en
dc.identifier.spage 26 en
dc.identifier.epage 32 en


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