Loading of intact rabbit erythrocytes with fluorophores and the enzyme pronase by means of electroporation

Haritou, M; Yova, D; Koutsouris, D; Loukas, S

dc.contributor.author	Haritou, M	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Koutsouris, D	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T01:13:53Z
dc.date.available	2014-03-01T01:13:53Z
dc.date.issued	1998	en
dc.identifier.issn	1386-0291	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12760
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0031738141&partnerID=40&md5=481dffe6092df29ab112c7e3853fb2c8	en
dc.subject	Electroporation	en
dc.subject	Erythrocytes	en
dc.subject	Loading	en
dc.subject	Pronase	en
dc.subject.classification	Hematology	en
dc.subject.classification	Peripheral Vascular Disease	en
dc.subject.other	pronase	en
dc.subject.other	animal cell	en
dc.subject.other	article	en
dc.subject.other	cell suspension	en
dc.subject.other	controlled study	en
dc.subject.other	electric field	en
dc.subject.other	electroporation	en
dc.subject.other	erythrocyte	en
dc.subject.other	erythrocyte membrane	en
dc.subject.other	nonhuman	en
dc.subject.other	rabbit	en
dc.subject.other	Animals	en
dc.subject.other	Cell Membrane Permeability	en
dc.subject.other	Electroporation	en
dc.subject.other	Erythrocyte Membrane	en
dc.subject.other	Erythrocytes	en
dc.subject.other	Fluoresceins	en
dc.subject.other	Fluorescent Dyes	en
dc.subject.other	Pronase	en
dc.subject.other	Rabbits	en
dc.title	Loading of intact rabbit erythrocytes with fluorophores and the enzyme pronase by means of electroporation	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1998	en
heal.abstract	The application of electric field pulses in cell suspensions is known to alter membrane integrity, resulting in increased membrane permeabilization. This field-induced membrane poration provides the means to load cells with a variety of external substances, useful for clinical applications. In this work, intact rabbit erythrocytes were successfully loaded with low molecular weight fluorescent probes and with the high molecular weight enzyme pronase, which has been shown to mimic the effects of insulin. Attachment of the enzyme onto the cell surface was also achieved by modifying the applied pulse parameters. Both applications were efficient and accompanied by high cell survival rates. In this way, biological carriers loaded with active substances were produced, offering the potentials for useful clinical applications, either for diagnostic or therapeutic purposes.	en
heal.publisher	IOS PRESS	en
heal.journalName	Clinical Hemorheology and Microcirculation	en
dc.identifier.isi	ISI:000077521500003	en
dc.identifier.volume	19	en
dc.identifier.issue	3	en
dc.identifier.spage	205	en
dc.identifier.epage	217	en