Modelling the effectiveness of a natural antimicrobial on Salmonella enteritidis as a function of concentration, temperature and pH, using conductance measurements

Koutsoumanis, K; Tassou, CC; Taoukis, PS; Nychas, G-JE

dc.contributor.author	Koutsoumanis, K	en
dc.contributor.author	Tassou, CC	en
dc.contributor.author	Taoukis, PS	en
dc.contributor.author	Nychas, G-JE	en
dc.date.accessioned	2014-03-01T01:13:55Z
dc.date.available	2014-03-01T01:13:55Z
dc.date.issued	1998	en
dc.identifier.issn	1364-5072	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12782
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Microbiology	en
dc.subject.other	oleuropein	en
dc.subject.other	phenol derivative	en
dc.subject.other	unclassified drug	en
dc.subject.other	antibiotic sensitivity	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	cell count	en
dc.subject.other	cell viability	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	drug efficacy	en
dc.subject.other	growth curve	en
dc.subject.other	nonhuman	en
dc.subject.other	ph	en
dc.subject.other	salmonella enteritidis	en
dc.subject.other	temperature	en
dc.subject.other	Colony Count, Microbial	en
dc.subject.other	Culture Media	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Electric Impedance	en
dc.subject.other	Food-Processing Industry	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Models, Biological	en
dc.subject.other	Pyrans	en
dc.subject.other	Salmonella enteritidis	en
dc.subject.other	Temperature	en
dc.title	Modelling the effectiveness of a natural antimicrobial on Salmonella enteritidis as a function of concentration, temperature and pH, using conductance measurements	en
heal.type	journalArticle	en
heal.identifier.primary	10.1046/j.1365-2672.1998.00433.x	en
heal.identifier.secondary	http://dx.doi.org/10.1046/j.1365-2672.1998.00433.x	en
heal.language	English	en
heal.publicationDate	1998	en
heal.abstract	The growth of Salmonella enteritidis in a brain heart infusion medium was monitored using the traditional viable count method and by conductance measurements using a Rabit impedance instrument. Growth curves (log(10) cfu ml(-1) vs time) at three different concentrations of oleuropein (0, 0.2 and 0.8 %), pH values in the range of 5-8 and incubation temperatures from 22 to 42 degrees C were modelled using the Gompertz equation. A good correlation between the maximum growth rate from the viable count method and the maximum slope of the conductance curve from the impedance instrument was established. Based on this correlation, the maximum specific growth rate of Salm. enteritidis was modelled as a function of the oleuropein concentration, initial pH values and the incubation temperature with. a quadratic equation, using a new, larger dataset of growth measurements by conductance. The developed model was validated by statistical comparison of predicted growth rates with growth rates determined by the viable count method, within the limits of the antimicrobial, pH and temperature domain.	en
heal.publisher	BLACKWELL SCIENCE LTD	en
heal.journalName	Journal of Applied Microbiology	en
dc.identifier.doi	10.1046/j.1365-2672.1998.00433.x	en
dc.identifier.isi	ISI:000075057100008	en
dc.identifier.volume	84	en
dc.identifier.issue	6	en
dc.identifier.spage	981	en
dc.identifier.epage	987	en