Studies on the solid-state production of thermostable endoxylanases from Thermoascus aurantiacus: Characterization of two isozymes

Kalogeris, E; Christakopoulos, P; Kekos, D; MacRis, BJ

dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	MacRis, BJ	en
dc.date.accessioned	2014-03-01T01:14:12Z
dc.date.available	2014-03-01T01:14:12Z
dc.date.issued	1998	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12923
dc.subject	Solid state cultivation	en
dc.subject	Thermoascus aurantiacus	en
dc.subject	Thermostability	en
dc.subject	Xylanase	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Carbon	en
dc.subject.other	Fungi	en
dc.subject.other	Moisture	en
dc.subject.other	Nitrogen	en
dc.subject.other	Particle size analysis	en
dc.subject.other	Separation	en
dc.subject.other	Thermodynamic stability	en
dc.subject.other	Isoelectric focusing	en
dc.subject.other	Solid state culture (SSC)	en
dc.subject.other	Xylanase	en
dc.subject.other	Xylosidase	en
dc.subject.other	Zymograms	en
dc.subject.other	Enzymes	en
dc.subject.other	carbon	en
dc.subject.other	hymecromone	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	xylose	en
dc.subject.other	article	en
dc.subject.other	enzyme assay	en
dc.subject.other	enzyme purification	en
dc.subject.other	enzyme specificity	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	fungus	en
dc.subject.other	hydrolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	Thermoascus	en
dc.subject.other	thermostability	en
dc.subject.other	Fungi	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Studies on the solid-state production of thermostable endoxylanases from Thermoascus aurantiacus: Characterization of two isozymes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0168-1656(97)00186-7	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0168-1656(97)00186-7	en
heal.language	English	en
heal.publicationDate	1998	en
heal.abstract	Production of xylanases by the thermophilic fungus Thermoascus aurantiacus under solid state culture (SSC) was enhanced by optimization of the type of carbon and nitrogen source, inoculum type, moisture level and particle size of the carbon source. Under these conditions, yields as high as 6193 U g-1 of carbon source were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major and two minor xylanases and one β-xylosidase. The major (xylanase I) and one of the minor (xylanase II) xylanases were separated and characterized. Both xylanases exhibited remarkable thermostability. Copyright (C) 1998 Elsevier Science B.V.Production of xylanases by the thermophilic fungus Thermoascus aurantiacus under solid state culture (SSC) was enhanced by optimization of the type of carbon and nitrogen source, inoculum type, moisture level and particle size of the carbon source. Under these conditions, yields as high as 6193 U g-1 of carbon source were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major and two minor xylanases and one β-xylosidase. The major (xylanase I) and one of the minor (xylanase II) xylanases were separated and characterized. Both xylanases exhibited remarkable thermostability.	en
heal.publisher	Elsevier Sci B.V., Amsterdam, Netherlands	en
heal.journalName	Journal of Biotechnology	en
dc.identifier.doi	10.1016/S0168-1656(97)00186-7	en
dc.identifier.isi	ISI:000073522800002	en
dc.identifier.volume	60	en
dc.identifier.issue	3	en
dc.identifier.spage	155	en
dc.identifier.epage	163	en