Studies on the synthesis of short-chain geranyl esters catalysed by fusarium oxysporum esterase in organic solvents

Stamatis, H; Christakopoulos, P; Kekos, D; Macris, BJ; Kolisis, FN

dc.contributor.author	Stamatis, H	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:14:12Z
dc.date.available	2014-03-01T01:14:12Z
dc.date.issued	1998	en
dc.identifier.issn	1381-1177	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/12924
dc.subject	Enzymatic synthesis	en
dc.subject	Fusarium oxysporum esterase	en
dc.subject	Geranyl ester	en
dc.subject	Organic solvent	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	Biosynthesis	en
dc.subject.other	Catalyst activity	en
dc.subject.other	Esterification	en
dc.subject.other	Esters	en
dc.subject.other	Organic solvents	en
dc.subject.other	Reaction kinetics	en
dc.subject.other	Substrates	en
dc.subject.other	Water	en
dc.subject.other	Esterase	en
dc.subject.other	Geranyl esters	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	esterase	en
dc.subject.other	article	en
dc.subject.other	catalysis	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme kinetics	en
dc.subject.other	enzyme mechanism	en
dc.subject.other	enzyme stability	en
dc.subject.other	esterification	en
dc.subject.other	fusarium oxysporum	en
dc.subject.other	nonhuman	en
dc.subject.other	reaction analysis	en
dc.title	Studies on the synthesis of short-chain geranyl esters catalysed by fusarium oxysporum esterase in organic solvents	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1381-1177(98)00003-4	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1381-1177(98)00003-4	en
heal.language	English	en
heal.publicationDate	1998	en
heal.abstract	A novel esterase isolated from Fusarium oxysporum was investigated for the synthesis of short-chain esters of geraniol by aicoholysis and direct esterification reactions in organic solvents. The enzyme was used as a dried powder (i.e., not immobilized). The reaction parameters affecting the enzyme behavior such as the nature of organic solvent and acyl donor, the concentration of substrates and the water activity of the system were studied. High yields (80-90%) were obtained by both approaches (alcoholysis and direct esterification) at low values of water activity (a(w) = 0.11) in n-hexane. The enzyme retain its catalytic activity even after fifth reuse in n-hexane at a(w) = 0.11, demonstrating its stability and efficiency under the conditions of this study.A novel esterase isolated from Fusarium oxysporum was investigated for the synthesis of short-chain esters of geraniol by alcoholysis and direct esterification reactions in organic solvents. The enzyme was used as a dried powder (i.e., not immobilized). The reaction parameters affecting the enzyme behavior such as the nature of organic solvent and acyl donor, the concentration of substrates and the water activity of the system were studied. High yields (80-90%) were obtained by both approaches (alcoholysis and direct esterification) at low values of water activity (aw = 0.11) in n-hexane. The enzyme retain its catalytic activity even after fifth reuse in n-hexane at aw = 0.11, demonstrating its stability and efficiency under the conditions of this study.	en
heal.publisher	Elsevier Sci B.V., Amsterdam, Netherlands	en
heal.journalName	Journal of Molecular Catalysis - B Enzymatic	en
dc.identifier.doi	10.1016/S1381-1177(98)00003-4	en
dc.identifier.isi	ISI:000074643300008	en
dc.identifier.volume	4	en
dc.identifier.issue	4	en
dc.identifier.spage	229	en
dc.identifier.epage	236	en