Enhanced acetyl esterase production by Fusarium oxysporum

Christakopoulos, P; Mamma, D; Kekos, D; Macris, BJ

dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Mamma, D	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:14:35Z
dc.date.available	2014-03-01T01:14:35Z
dc.date.issued	1999	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13155
dc.subject	Acetyl (xylan) esterase production, Fusarium oxysporum	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	4 nitrophenylacetate	en
dc.subject.other	acetyl esterase	en
dc.subject.other	acetylxylan	en
dc.subject.other	deacetylation	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	growth regulation	en
dc.subject.other	microbe metabolism	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Enhanced acetyl esterase production by Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1008936204368	en
heal.identifier.secondary	http://dx.doi.org/10.1023/A:1008936204368	en
heal.language	English	en
heal.publicationDate	1999	en
heal.abstract	Production of acetyl esterase (EC 3.1.1.6) by Fusarium oxysporum strain F3 was enhanced by optimization of growth conditions. Under optimal conditions, activities as high as 0.89 U/ml of culture medium were obtained. The culture filtrate was equally active on p-nitrophenyl acetate and acetylxylan. The enzyme produced 71% deacetylation of acetylxylan in 2 h at 40 degrees C. Activity was optimized at pH 6.5 and at 55 degrees C. The respective K-m values for p-nitrophenyl acetate and acetylxylan were 0.25 mM and 1.05% (w/v) and the V-m values were 0.65 and 0.43 mu mol acetate/min/mg protein.	en
heal.publisher	KLUWER ACADEMIC PUBL	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1023/A:1008936204368	en
dc.identifier.isi	ISI:000082822900006	en
dc.identifier.volume	15	en
dc.identifier.issue	4	en
dc.identifier.spage	443	en
dc.identifier.epage	446	en