In vitro evaluation of the genotoxic and clastogenic potential of photodynamic therapy

Halkiotis, K; Yova, D; Pantelias, G

dc.contributor.author	Halkiotis, K	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Pantelias, G	en
dc.date.accessioned	2014-03-01T01:14:43Z
dc.date.available	2014-03-01T01:14:43Z
dc.date.issued	1999	en
dc.identifier.issn	0267-8357	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13199
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0032976143&partnerID=40&md5=1eceb235137dd9004a15e863937dede4	en
dc.subject.classification	Genetics & Heredity	en
dc.subject.classification	Toxicology	en
dc.subject.other	photosensitizing agent	en
dc.subject.other	phthalocyanine zinc	en
dc.subject.other	article	en
dc.subject.other	cell killing	en
dc.subject.other	cell line	en
dc.subject.other	cell membrane	en
dc.subject.other	chromosome aberration	en
dc.subject.other	chromosome analysis	en
dc.subject.other	chromosome damage	en
dc.subject.other	controlled study	en
dc.subject.other	genotoxicity	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	laser	en
dc.subject.other	mitochondrion	en
dc.subject.other	pancreas cancer	en
dc.subject.other	photodynamic therapy	en
dc.subject.other	priority journal	en
dc.subject.other	radiation dose	en
dc.subject.other	sister chromatid exchange	en
dc.subject.other	Adenocarcinoma	en
dc.subject.other	Cell Death	en
dc.subject.other	Cell Survival	en
dc.subject.other	Chromosome Aberrations	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Dose-Response Relationship, Radiation	en
dc.subject.other	Humans	en
dc.subject.other	Indoles	en
dc.subject.other	Lasers	en
dc.subject.other	Mutagenesis	en
dc.subject.other	Organometallic Compounds	en
dc.subject.other	Pancreas	en
dc.subject.other	Photochemotherapy	en
dc.subject.other	Photosensitizing Agents	en
dc.subject.other	Sister Chromatid Exchange	en
dc.subject.other	Tumor Cells, Cultured	en
dc.subject.other	Tumor Stem Cell Assay	en
dc.subject.other	Agouti paca	en
dc.title	In vitro evaluation of the genotoxic and clastogenic potential of photodynamic therapy	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1999	en
heal.abstract	Photodynamic therapy (PDT) was recently introduced in clinical practice for the management of cancer. As far as PDT relies on the combined action of a photosensitizer and a laser source, there is a need to evaluate the genotoxic and mutagenic potential of this treatment modality. This paper reports the effects of various photosensitizer and photo-irradiation doses on lethality to the MIA PaCa cell line using ZnPcS4 as the photosensitizer, The sister chromatid exchange (SCE) assay was used to evaluate the genotoxicity of various photosensitizer and photoirradiation doses. Also, chromosomal aberrations at various time intervals post-irradiation were evaluated. The results showed that a combination of 3 J/cm(2) irradiance with 5 mu M ZnPcS4 concentration leads to the LD90 72 h post-irradiation. Eight days post-irradiation the LD90 level was achieved using a light dose of 3 J/cm(2), independent of ZnPcS4 concentration. The SCE assay showed that cells treated with various light and drug doses presented no genotoxic potential, as SCE levels were not different from untreated (control) cells, Chromosomal analysis after PDT treatment at various time intervals post-irradiation showed that there was no significant chromosomal damage in cells treated photodynamically compared with untreated controls. The results show that the cell killing mechanism after PDT is not at the chromosome level, but may be at a different cellular level, such as plasma membranes, mitochondria, etc.	en
heal.publisher	OXFORD UNIV PRESS	en
heal.journalName	Mutagenesis	en
dc.identifier.isi	ISI:000079269100005	en
dc.identifier.volume	14	en
dc.identifier.issue	2	en
dc.identifier.spage	193	en
dc.identifier.epage	198	en