The 'behaviour' of five metal biosorbing and bioprecipitating bacterial strains, inoculated in a moving-bed sand filter

Pernfuss, B; Ebner, C; Pumpel, T; Diels, L; Macaskie, L; Tsezos, M; Keszthelyi, Z; Glombitza, F

dc.contributor.author	Pernfuss, B	en
dc.contributor.author	Ebner, C	en
dc.contributor.author	Pumpel, T	en
dc.contributor.author	Diels, L	en
dc.contributor.author	Macaskie, L	en
dc.contributor.author	Tsezos, M	en
dc.contributor.author	Keszthelyi, Z	en
dc.contributor.author	Glombitza, F	en
dc.date.accessioned	2014-03-01T01:15:17Z
dc.date.available	2014-03-01T01:15:17Z
dc.date.issued	1999	en
dc.identifier.issn	15724409	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13412
dc.title	The 'behaviour' of five metal biosorbing and bioprecipitating bacterial strains, inoculated in a moving-bed sand filter	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1572-4409(99)80127-8	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1572-4409(99)80127-8	en
heal.publicationDate	1999	en
heal.abstract	In the course of a RTD-project* we tried to remove nickel from a rinsing water of a plating company in Vienna. To this purpose a moving-bed 'AstraSand' filter was inoculated with a mixture of five well investigated (Brite-Euram 5350) bacterial strains with a high potential to biosorb and bioprecipitate heavy, metals. Three of the five bacterial strains (Pseudomonas mendocina As 302, Arthrobacter sp. BP 7/26, Alcaligenes eutrophus CH 34, Pseudomonas fluorescens K 1/8a and Methylobacillus sp. MB 127) could be adapted separately to the waste water before inoculation was carried out using a mixed culture. On the basis of morphological and physiological characteristics of the bacteria-four of the strains stain gram-negative, one gram-positive; the strains use different carbon sources and some of them bear heavy metal resistances-selective agar media were collated to re-isolate the strains out of the mixture. With this simple method it could be shown that one of the strains, which could not be preadapted to the rinsing water by separate cultivation, was able to survive in the waste water when it was precultured together with the other strains.*Brite-Euram BRPR-CT96-0172. After the inoculation of the non-sterile moving-bed reactor and during its operation the proliferation of autochthonous microorganisms could be observed in the waste water in a high density. Periodically the composition of the biofilm, grown on the sand particles, and of the planktonic microorganisms was investigated. The influence of continous sand regeneration on the growth of the biofilm was documented regarding different operation models. Eutrophic-, oligotrophic-, Cd-resistant-, Ni-resistant-, As-resistant- and methylo Pseudomonas mendocina AS 302 and Pseudomonas fluorescens K 1/8a), and the family Nocardiaceae (like Arthrobacter sp. BP 7/26). Several re-isolates were related to Acinetobacter calcoaceticus BV ALC. Colonies of this species were found on agar plates containing methanol (No. 22) on nickel containing agar plates (No. 26) and, very frequently, on the medium made of the nickel containing waste water (No. 33). Thus, it can be concluded that Acinetobacter calcoaceticus BV ALC as an autochthonous strain takes part in the biofilm of the moving-bed filter for the waste water treatment of the plating company 'Skolnik'. © 1999 Elsevier B.V. All rights reserved.	en
heal.journalName	Process Metallurgy	en
dc.identifier.doi	10.1016/S1572-4409(99)80127-8	en
dc.identifier.volume	9	en
dc.identifier.issue	C	en
dc.identifier.spage	373	en
dc.identifier.epage	382	en