dc.contributor.author |
Haritou, M |
en |
dc.contributor.author |
Yova, D |
en |
dc.contributor.author |
Loukas, S |
en |
dc.date.accessioned |
2014-03-01T01:15:27Z |
|
dc.date.available |
2014-03-01T01:15:27Z |
|
dc.date.issued |
2000 |
en |
dc.identifier.issn |
0302-4598 |
en |
dc.identifier.uri |
https://dspace.lib.ntua.gr/xmlui/handle/123456789/13516 |
|
dc.subject |
electrofusion |
en |
dc.subject |
erythrocytes |
en |
dc.subject |
fusion facilitating agents |
en |
dc.subject.classification |
Biochemistry & Molecular Biology |
en |
dc.subject.classification |
Biology |
en |
dc.subject.classification |
Biophysics |
en |
dc.subject.classification |
Electrochemistry |
en |
dc.subject.other |
INDUCED CELL-FUSION |
en |
dc.subject.other |
MAMMALIAN-CELLS |
en |
dc.subject.other |
ELECTROFUSION |
en |
dc.subject.other |
MEMBRANE |
en |
dc.subject.other |
GHOSTS |
en |
dc.subject.other |
ELECTROPERMEABILIZATION |
en |
dc.subject.other |
PROTOPLASTS |
en |
dc.subject.other |
HEMOLYSIS |
en |
dc.subject.other |
PROTEINS |
en |
dc.subject.other |
ANTIBODY |
en |
dc.title |
Agents facilitating the electric field-induced fusion of intact rabbit erythrocytes |
en |
heal.type |
journalArticle |
en |
heal.identifier.primary |
10.1016/S0302-4598(00)00105-7 |
en |
heal.identifier.secondary |
http://dx.doi.org/10.1016/S0302-4598(00)00105-7 |
en |
heal.language |
English |
en |
heal.publicationDate |
2000 |
en |
heal.abstract |
In the recent years, the role of specific membrane active agents in the electrofusion process has started to draw attention, and it has been found that the presence of various substances in the cell medium can affect the fusion process either in a positive or negative way. In this work, the effect of several proteins, bivalent cations and antibiotics was tested with respect to their ability to protect intact erythrocytes from hemolysis and facilitate the fusion process. The effect of different sugars was also studied. Among the different proteins, pronase acid proteinase were found to be the most effective. With respect to bivalent cations, Ca2+ and Mn2+ were more effective while Mg2+ was less important. From the antibiotics, penicillin caused a negative effect while streptomycin acted positively. Finally, glucose medium was found to be the most effective compared to all sugars tested. The results indicated that there are strong differentiations of the induced effects caused by each substance, and some possible mechanisms of action of these agents on the erythrocyte membrane were discussed. (C) 2000 Elsevier Science S.A, All rights reserved. |
en |
heal.publisher |
ELSEVIER SCIENCE SA |
en |
heal.journalName |
BIOELECTROCHEMISTRY |
en |
dc.identifier.doi |
10.1016/S0302-4598(00)00105-7 |
en |
dc.identifier.isi |
ISI:000167099800014 |
en |
dc.identifier.volume |
52 |
en |
dc.identifier.issue |
2 |
en |
dc.identifier.spage |
229 |
en |
dc.identifier.epage |
238 |
en |