Agents facilitating the electric field-induced fusion of intact rabbit erythrocytes

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dc.contributor.author Haritou, M en
dc.contributor.author Yova, D en
dc.contributor.author Loukas, S en
dc.date.accessioned 2014-03-01T01:15:27Z
dc.date.available 2014-03-01T01:15:27Z
dc.date.issued 2000 en
dc.identifier.issn 0302-4598 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/13516
dc.subject electrofusion en
dc.subject erythrocytes en
dc.subject fusion facilitating agents en
dc.subject.classification Biochemistry & Molecular Biology en
dc.subject.classification Biology en
dc.subject.classification Biophysics en
dc.subject.classification Electrochemistry en
dc.subject.other INDUCED CELL-FUSION en
dc.subject.other MAMMALIAN-CELLS en
dc.subject.other ELECTROFUSION en
dc.subject.other MEMBRANE en
dc.subject.other GHOSTS en
dc.subject.other PROTOPLASTS en
dc.subject.other HEMOLYSIS en
dc.subject.other PROTEINS en
dc.subject.other ANTIBODY en
dc.title Agents facilitating the electric field-induced fusion of intact rabbit erythrocytes en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0302-4598(00)00105-7 en
heal.identifier.secondary http://dx.doi.org/10.1016/S0302-4598(00)00105-7 en
heal.language English en
heal.publicationDate 2000 en
heal.abstract In the recent years, the role of specific membrane active agents in the electrofusion process has started to draw attention, and it has been found that the presence of various substances in the cell medium can affect the fusion process either in a positive or negative way. In this work, the effect of several proteins, bivalent cations and antibiotics was tested with respect to their ability to protect intact erythrocytes from hemolysis and facilitate the fusion process. The effect of different sugars was also studied. Among the different proteins, pronase acid proteinase were found to be the most effective. With respect to bivalent cations, Ca2+ and Mn2+ were more effective while Mg2+ was less important. From the antibiotics, penicillin caused a negative effect while streptomycin acted positively. Finally, glucose medium was found to be the most effective compared to all sugars tested. The results indicated that there are strong differentiations of the induced effects caused by each substance, and some possible mechanisms of action of these agents on the erythrocyte membrane were discussed. (C) 2000 Elsevier Science S.A, All rights reserved. en
heal.publisher ELSEVIER SCIENCE SA en
dc.identifier.doi 10.1016/S0302-4598(00)00105-7 en
dc.identifier.isi ISI:000167099800014 en
dc.identifier.volume 52 en
dc.identifier.issue 2 en
dc.identifier.spage 229 en
dc.identifier.epage 238 en

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