Agents facilitating the electric field-induced fusion of intact rabbit erythrocytes

Haritou, M; Yova, D; Loukas, S

dc.contributor.author	Haritou, M	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T01:15:27Z
dc.date.available	2014-03-01T01:15:27Z
dc.date.issued	2000	en
dc.identifier.issn	0302-4598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13516
dc.subject	electrofusion	en
dc.subject	erythrocytes	en
dc.subject	fusion facilitating agents	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biology	en
dc.subject.classification	Biophysics	en
dc.subject.classification	Electrochemistry	en
dc.subject.other	INDUCED CELL-FUSION	en
dc.subject.other	MAMMALIAN-CELLS	en
dc.subject.other	ELECTROFUSION	en
dc.subject.other	MEMBRANE	en
dc.subject.other	GHOSTS	en
dc.subject.other	ELECTROPERMEABILIZATION	en
dc.subject.other	PROTOPLASTS	en
dc.subject.other	HEMOLYSIS	en
dc.subject.other	PROTEINS	en
dc.subject.other	ANTIBODY	en
dc.title	Agents facilitating the electric field-induced fusion of intact rabbit erythrocytes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0302-4598(00)00105-7	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0302-4598(00)00105-7	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	In the recent years, the role of specific membrane active agents in the electrofusion process has started to draw attention, and it has been found that the presence of various substances in the cell medium can affect the fusion process either in a positive or negative way. In this work, the effect of several proteins, bivalent cations and antibiotics was tested with respect to their ability to protect intact erythrocytes from hemolysis and facilitate the fusion process. The effect of different sugars was also studied. Among the different proteins, pronase acid proteinase were found to be the most effective. With respect to bivalent cations, Ca2+ and Mn2+ were more effective while Mg2+ was less important. From the antibiotics, penicillin caused a negative effect while streptomycin acted positively. Finally, glucose medium was found to be the most effective compared to all sugars tested. The results indicated that there are strong differentiations of the induced effects caused by each substance, and some possible mechanisms of action of these agents on the erythrocyte membrane were discussed. (C) 2000 Elsevier Science S.A, All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE SA	en
heal.journalName	BIOELECTROCHEMISTRY	en
dc.identifier.doi	10.1016/S0302-4598(00)00105-7	en
dc.identifier.isi	ISI:000167099800014	en
dc.identifier.volume	52	en
dc.identifier.issue	2	en
dc.identifier.spage	229	en
dc.identifier.epage	238	en