Effects of purified endo-beta-1,4-xylanases of family 10 and 11 and acetyl xylan esterases on eucalypt sulfite dissolving pulp

Christov, L; Biely, P; Kalogeris, E; Christakopoulos, P; Prior, BA; Bhat, MK

dc.contributor.author	Christov, L	en
dc.contributor.author	Biely, P	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Prior, BA	en
dc.contributor.author	Bhat, MK	en
dc.date.accessioned	2014-03-01T01:15:34Z
dc.date.available	2014-03-01T01:15:34Z
dc.date.issued	2000	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13601
dc.subject	endo-xylanases of family 10 and 11	en
dc.subject	acetyl xylan esterase	en
dc.subject	dissolving pulp	en
dc.subject	degradation products	en
dc.subject	biobleaching	en
dc.subject	xylan hydrolysis	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	TRICHODERMA-REESEI	en
dc.subject.other	SCHIZOPHYLLUM-COMMUNE	en
dc.subject.other	STREPTOMYCES-LIVIDANS	en
dc.subject.other	CATALYTIC PROPERTIES	en
dc.subject.other	ENZYMATIC REMOVAL	en
dc.subject.other	KRAFT PULP	en
dc.subject.other	PRETREATMENT	en
dc.subject.other	PURIFICATION	en
dc.subject.other	BLEACHABILITY	en
dc.subject.other	HEMICELLULOSE	en
dc.title	Effects of purified endo-beta-1,4-xylanases of family 10 and 11 and acetyl xylan esterases on eucalypt sulfite dissolving pulp	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0168-1656(00)00324-2	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0168-1656(00)00324-2	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	Sulfite dissolving pulp from Eucalyptus grandis contained approximately 3.8% O-acetyl-4-O-methylglucuronoxylan with a molar ratio of xylose:4-O-methylglucuronic acid:acetyl group close to 13.6:1:6.2. The effects produced by purified endo-xylanases from two different glycosyl hydrolase families (family 10 and 11) as well as acetyl xylan esterases were examined and assessed on pulp in relation to their bleaching abilities. The purified endo-xylanases hpdrolyzed only a limited portion (less than 30%) of the acetylglucuronoxylan present in the pulp. The enzymes of family 10 produced acetylated xylobiose and xylotriose whereas acetylated xylobiose was not observed among the products released from the pulp by the family 11 xylanases. The esterases however were not capable of deacetylating the acetylated aldouronic acids generated by the xylanases. Regardless of the different mode of action of the endo-xylanases on dissolving pulp, their effect on pulp bleaching was not related to the amount and nature of sugars generated or the glycosyl hydrolase family. No additional brightness gain was obtained when endo-xylanases were used in conjunction with acetyl xylan esterases, suggesting that the latter do not play an important role in biobleaching of eucalypt sulfite dissolving pulps. (C) 2000 Elsevier Science B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	JOURNAL OF BIOTECHNOLOGY	en
dc.identifier.doi	10.1016/S0168-1656(00)00324-2	en
dc.identifier.isi	ISI:000089668500006	en
dc.identifier.volume	83	en
dc.identifier.issue	3	en
dc.identifier.spage	231	en
dc.identifier.epage	244	en