Hemicellulolytic activity of Fusarium oxysporum grown on sugar beet pulp. Production of extracellular arabinanase

Cheilas, T; Stoupis, T; Christakopoulos, P; Katapodis, P; Mamma, D; Hatzinikolaou, DG; Kekos, D; Macris, BJ

dc.contributor.author	Cheilas, T	en
dc.contributor.author	Stoupis, T	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Mamma, D	en
dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:15:38Z
dc.date.available	2014-03-01T01:15:38Z
dc.date.issued	2000	en
dc.identifier.issn	0032-9592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/13638
dc.subject	arabinanase production	en
dc.subject	Fusarium oxysporum	en
dc.subject	sugar-beet pulp	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Engineering, Chemical	en
dc.subject.other	ALPHA-L-ARABINOFURANOSIDASE	en
dc.subject.other	PURIFICATION	en
dc.subject.other	XYLANASE	en
dc.subject.other	CULTURE	en
dc.subject.other	ENDO-1,4-BETA-D-GLUCANASE	en
dc.subject.other	SACCHARIFICATION	en
dc.subject.other	CELLULASE	en
dc.subject.other	PECTINASE	en
dc.subject.other	ENZYMES	en
dc.subject.other	MODE	en
dc.title	Hemicellulolytic activity of Fusarium oxysporum grown on sugar beet pulp. Production of extracellular arabinanase	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0032-9592(99)00103-X	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0032-9592(99)00103-X	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	Fusarium oxysporum F3 exhibited hemicellulolytic enzymic activity when grown on sugar beet pulp, a by-product of the sugar industry. The growth medium was specifically optimised for enhanced production of extracellular arabinanase. The optimum medium contained sugar beet pulp (4%, w/v) and corn steep liquor (6%, v/v) as carbon and nitrogen sources, respectively. Arabinanase activity as high as 0.25 U/ml of culture was obtained, which compared favourably to those reported for other microorganisms. Optimal arabinanase activity was observed at pH 6-7 and 50 degrees C. Investigation of the degradation of the main components of sugar beet pulp showed that arabinose containing polysaccharides and pectin were first degraded, followed by the glucose-containing polysaccharides. (C) 2000 Elsevier Science Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	PROCESS BIOCHEMISTRY	en
dc.identifier.doi	10.1016/S0032-9592(99)00103-X	en
dc.identifier.isi	ISI:000084040400003	en
dc.identifier.volume	35	en
dc.identifier.issue	6	en
dc.identifier.spage	557	en
dc.identifier.epage	561	en