Determination of ETU in tomatoes and tomato products by HPLC-PDA. Evaluation of cleanup procedures

Kontou, S; Tsipi, D; Oreopoulou, V; Tzia, C

dc.contributor.author	Kontou, S	en
dc.contributor.author	Tsipi, D	en
dc.contributor.author	Oreopoulou, V	en
dc.contributor.author	Tzia, C	en
dc.date.accessioned	2014-03-01T01:16:23Z
dc.date.available	2014-03-01T01:16:23Z
dc.date.issued	2001	en
dc.identifier.issn	0021-8561	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14022
dc.subject	Ethylenethiourea	en
dc.subject	HPLC-PDA	en
dc.subject	Pesticide residues	en
dc.subject	Solid-matrix l-l partitioning	en
dc.subject	Tomato products	en
dc.subject.classification	Agriculture, Multidisciplinary	en
dc.subject.classification	Chemistry, Applied	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	2 imidazolidinethione	en
dc.subject.other	n,n' ethylenebis(dithiocarbamic acid) derivative	en
dc.subject.other	pesticide residue	en
dc.subject.other	article	en
dc.subject.other	diagnostic accuracy	en
dc.subject.other	drug determination	en
dc.subject.other	food analysis	en
dc.subject.other	food contamination	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	liquid liquid extraction	en
dc.subject.other	reversed phase high performance liquid chromatography	en
dc.subject.other	sensitivity and specificity	en
dc.subject.other	solid phase extraction	en
dc.subject.other	tomato	en
dc.subject.other	Chromatography, High Pressure Liquid	en
dc.subject.other	Ethylenebis(dithiocarbamates)	en
dc.subject.other	Ethylenethiourea	en
dc.subject.other	Food Handling	en
dc.subject.other	Fungicides, Industrial	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Pesticide Residues	en
dc.subject.other	Reproducibility of Results	en
dc.subject.other	Sensitivity and Specificity	en
dc.subject.other	Lycopersicon esculentum	en
dc.title	Determination of ETU in tomatoes and tomato products by HPLC-PDA. Evaluation of cleanup procedures	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/jf000116e	en
heal.identifier.secondary	http://dx.doi.org/10.1021/jf000116e	en
heal.language	English	en
heal.publicationDate	2001	en
heal.abstract	HPLC-PDA method for the determination of ethylenethiourea (ETU), the main degradation product of the organic fungicides ethylene bis(dithiocarbamate)s (EBDCs), in tomatoes and tomato products is reported. Solid-matrix liquid-liquid (l-l) partitioning and separatory funnel l-l partitioning for the cleanup were examined. The effect of salt addition, pH, and phase ratio on analyte recovery at the cleanup step was studied. It was found that solid-matrix l-l partitioning afforded higher precision and more selective separation of the analyte. According to the method proposed, the samples were extracted with methanol/water (3:1, v/v) and cleaned up on an Extrelut 20 column. ETU was eluted with dichloromethane and separated on a reversed phase HPLC column. For tomato products with degrees Brix > 20 further purification through silica cartridge was adopted. The method was validated over the following ranges of concentrations: 0.01-0.5 mg/kg for tomatoes, 0.01-0.1 mg/kg for tomato juice, and 0.05-0.25 mg/kg for tomato paste. The accuracy (recoveries > 70%) and the precision obtained (%RSD < 10%) were satisfactory.	en
heal.publisher	AMER CHEMICAL SOC	en
heal.journalName	Journal of Agricultural and Food Chemistry	en
dc.identifier.doi	10.1021/jf000116e	en
dc.identifier.isi	ISI:000168967400003	en
dc.identifier.volume	49	en
dc.identifier.issue	3	en
dc.identifier.spage	1090	en
dc.identifier.epage	1097	en