Dielectric and UV spectrophotometric study of physicochemical effects of ionizing radiation on mammalian macromolecular DNA

Georgakilas, AG; Konsta, AA; Sideris, EG; Sakelliou, L

dc.contributor.author	Georgakilas, AG	en
dc.contributor.author	Konsta, AA	en
dc.contributor.author	Sideris, EG	en
dc.contributor.author	Sakelliou, L	en
dc.date.accessioned	2014-03-01T01:16:24Z
dc.date.available	2014-03-01T01:16:24Z
dc.date.issued	2001	en
dc.identifier.issn	1070-9878	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14035
dc.subject	Dielectric Relaxation	en
dc.subject	Ionizing Radiation	en
dc.subject	Pulse Field Gel Electrophoresis	en
dc.subject	Radiation Dose	en
dc.subject	Thermal Stability	en
dc.subject	Calf Thymus	en
dc.subject	Low Dose	en
dc.subject	Molecular Weight	en
dc.subject.classification	Engineering, Electrical & Electronic	en
dc.subject.classification	Physics, Applied	en
dc.subject.other	Alpha particles	en
dc.subject.other	Dielectric relaxation	en
dc.subject.other	DNA	en
dc.subject.other	Electrophoresis	en
dc.subject.other	Gamma rays	en
dc.subject.other	Irradiation	en
dc.subject.other	Molecular weight	en
dc.subject.other	Phase transitions	en
dc.subject.other	Spectrophotometry	en
dc.subject.other	Thermal conductivity of solids	en
dc.subject.other	Thermodynamics	en
dc.subject.other	Physiochemical effects	en
dc.subject.other	Pulse field gel electrophoresis (PFGE)	en
dc.subject.other	Macromolecules	en
dc.title	Dielectric and UV spectrophotometric study of physicochemical effects of ionizing radiation on mammalian macromolecular DNA	en
heal.type	journalArticle	en
heal.identifier.primary	10.1109/94.933382	en
heal.identifier.secondary	http://dx.doi.org/10.1109/94.933382	en
heal.language	English	en
heal.publicationDate	2001	en
heal.abstract	The purpose of this work was the comparative investigation of physicochemical effects induced by gamma and alpha radiation on the thermal denaturation dynamics of native macromolecular calf thymus DNA, combining dielectric relaxation spectroscopy (DRS) and vv thermal transition spectrophotometry (TTS), while the apparent molecular weight (Mw) distribution of the DNA macromolecules was measured by pulse field gel electrophoresis (PFGE). A DNA thermal stability enhancement over the non-irradiated samples was observed at radiation doses < 10 Gy for gamma rays and 32 Gy for alpha particles, attributed to the development of intra and/or intermolecular DNA-DNA interactions, whereas at higher irradiation doses, the expected decrease of thermal stability was observed. Moreover, in all studied cases, the recorded conductivity changes were found to precede by 2 to 3 degreesC those of UV TTS absorbance changes, a fact indicative of a multi-stage process for DNA thermal transition. Finally, the displacement of the low dose region limit towards higher doses in the case of alpha particles, confirm earlier results attesting higher yields of DNA strand breaks induced by gamma rays compared to alpha particles.	en
heal.publisher	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC	en
heal.journalName	IEEE Transactions on Dielectrics and Electrical Insulation	en
dc.identifier.doi	10.1109/94.933382	en
dc.identifier.isi	ISI:000169815200044	en
dc.identifier.volume	8	en
dc.identifier.issue	3	en
dc.identifier.spage	549	en
dc.identifier.epage	554	en