Enzymatic acylation of hydroxypropyl cellulose in organic media and determination of ester formation by Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy

Sereti, V; Stamatis, H; Pappas, C; Polissiou, M; Kolisis Biotechnology Laboratory, FN

dc.contributor.author	Sereti, V	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Pappas, C	en
dc.contributor.author	Polissiou, M	en
dc.contributor.author	Kolisis Biotechnology Laboratory, FN	en
dc.date.accessioned	2014-03-01T01:16:33Z
dc.date.available	2014-03-01T01:16:33Z
dc.date.issued	2001	en
dc.identifier.issn	0006-3592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14079
dc.subject	Cellulose	en
dc.subject	Esterification	en
dc.subject	Fourier transform-infrared (FT-IR) spectroscopy	en
dc.subject	Hydroxypropyl cellulose	en
dc.subject	Lipase	en
dc.subject	Organic media	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	ester	en
dc.subject.other	hydroxypropylcellulose	en
dc.subject.other	organic compound	en
dc.subject.other	absorption	en
dc.subject.other	acylation	en
dc.subject.other	article	en
dc.subject.other	biodegradation	en
dc.subject.other	Candida antarctica	en
dc.subject.other	catalysis	en
dc.subject.other	diffuse reflectance spectroscopy	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	esterification	en
dc.subject.other	Fourier transformation	en
dc.subject.other	nonhuman	en
dc.subject.other	protein content	en
dc.subject.other	reaction analysis	en
dc.subject.other	stereochemistry	en
dc.subject.other	Acylation	en
dc.subject.other	Candida	en
dc.subject.other	Carboxymethylcellulose	en
dc.subject.other	Catalysis	en
dc.subject.other	Cellulose	en
dc.subject.other	Enzymes, Immobilized	en
dc.subject.other	Esters	en
dc.subject.other	Fungal Proteins	en
dc.subject.other	Lipase	en
dc.subject.other	Rhizomucor	en
dc.subject.other	Solvents	en
dc.subject.other	Spectroscopy, Fourier Transform Infrared	en
dc.subject.other	tert-Butyl Alcohol	en
dc.subject.other	Viscosity	en
dc.subject.other	Candida antarctica	en
dc.title	Enzymatic acylation of hydroxypropyl cellulose in organic media and determination of ester formation by Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/1097-0290(20010220)72:4<495::AID-BIT1012>3.0.CO;2-T	en
heal.identifier.secondary	http://dx.doi.org/10.1002/1097-0290(20010220)72:4<495::AID-BIT1012>3.0.CO;2-T	en
heal.language	English	en
heal.publicationDate	2001	en
heal.abstract	Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier transform-infrared (FT-IR) spectroscopy was used for determination of ester content on hydroxypropyl cellulose. Specifically, a linear relationship was established between the peak height assigned to the absorption of the esterified carboxyl groups of the cellulose and the ester content. At optimum reaction conditions, the ester content on the hydroxypropyl cellulose was about 11%. (C) 2001 John Wiley & Sons, Inc.	en
heal.publisher	JOHN WILEY & SONS INC	en
heal.journalName	Biotechnology and Bioengineering	en
dc.identifier.doi	10.1002/1097-0290(20010220)72:4<495::AID-BIT1012>3.0.CO;2-T	en
dc.identifier.isi	ISI:000166774900013	en
dc.identifier.volume	72	en
dc.identifier.issue	4	en
dc.identifier.spage	495	en
dc.identifier.epage	500	en