Microscopical examination of the localisation patterns of two novel rhodamine derivatives in normal and neoplastic colonic mucosa

Atlamazoglou, V; Yova, D; Kavantzas, N; Loukas, S

dc.contributor.author	Atlamazoglou, V	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Kavantzas, N	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T01:16:44Z
dc.date.available	2014-03-01T01:16:44Z
dc.date.issued	2001	en
dc.identifier.issn	0268-8921	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14192
dc.subject	Colon cancer	en
dc.subject	Fluorescence microscopy	en
dc.subject	Lectins	en
dc.subject	Mucin histochemistry	en
dc.subject	Rhodamine derivatives	en
dc.subject.classification	Engineering, Biomedical	en
dc.subject.classification	Surgery	en
dc.subject.other	alexa 350	en
dc.subject.other	benzeneboronic acid	en
dc.subject.other	boronic acid derivative	en
dc.subject.other	carminic acid	en
dc.subject.other	concanavalin A	en
dc.subject.other	dye	en
dc.subject.other	leucine	en
dc.subject.other	mucicarmine	en
dc.subject.other	mucin	en
dc.subject.other	n acetylglucosamine	en
dc.subject.other	rhodamine	en
dc.subject.other	rhodamine B	en
dc.subject.other	sialic acid	en
dc.subject.other	unclassified drug	en
dc.subject.other	wheat germ agglutinin	en
dc.subject.other	article	en
dc.subject.other	biochemistry	en
dc.subject.other	camera	en
dc.subject.other	cancer diagnosis	en
dc.subject.other	colon cancer	en
dc.subject.other	diagnostic accuracy	en
dc.subject.other	diagnostic value	en
dc.subject.other	differential diagnosis	en
dc.subject.other	drug distribution	en
dc.subject.other	drug selectivity	en
dc.subject.other	drug synthesis	en
dc.subject.other	fluorescence microscopy	en
dc.subject.other	histochemistry	en
dc.subject.other	human	en
dc.subject.other	human tissue	en
dc.subject.other	intestine crypt	en
dc.subject.other	priority journal	en
dc.subject.other	Carmine	en
dc.subject.other	Colonic Neoplasms	en
dc.subject.other	Coloring Agents	en
dc.subject.other	Concanavalin A	en
dc.subject.other	Humans	en
dc.subject.other	Indicators and Reagents	en
dc.subject.other	Microscopy, Fluorescence	en
dc.subject.other	Rhodamines	en
dc.subject.other	Wheat Germ Agglutinins	en
dc.title	Microscopical examination of the localisation patterns of two novel rhodamine derivatives in normal and neoplastic colonic mucosa	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/PL00011361	en
heal.identifier.secondary	http://dx.doi.org/10.1007/PL00011361	en
heal.language	English	en
heal.publicationDate	2001	en
heal.abstract	Tissue characterisation by fluorescence imaging, using exogenous fluorophores, is a promising method for cancer detection. Histochemical alterations in the composition of mucins, when neoplastic transformations occur, could be exploited to derive more selective fluoroprobes indicative of early malignant transformation. The aim of this work was to develop and examine tumour selective fluoroprobes for colon cancer diagnosis, as well as to determine the morphological components where selective dye accumulation has occurred. Two novel fluoroprobes: rhodamine B-L-leucine amide and rhodamine B-phenylboronic acid were synthesised and examined together with Mayer's mucicarmine, alexa, 350-wheat germ agglutinin (WGA) and tetramethyl rhodamine-concanavalin A (ConA). Fluorescence microscopy studies were performed with deparaffinised human colon sections, using an epifluorescence microscope equipped with a colour CCD camera. The intense accumulation of the novel fluoroprobes was localised in the amorphous material in the lumen of neoplastic crypts. To gain insight into the localisation patterns, mucicarmine, alexa. 350-WGA and tetramethyl rhodamine-ConA were used. Alexa 350-WGA reacted primarily with mucin secreted in the malignant crypt lumen suggesting that this material is rich in sialic acid and N-acetylglucosaminyl residues. These derivatives clearly and consistently distinguished non-neoplastic from neoplastic human colon tissue sections. The intense accumulation at the altered mucins indicates that they could be used as fluoroprobes of biochemical alterations for carcinoma detection.	en
heal.publisher	SPRINGER-VERLAG LONDON LTD	en
heal.journalName	Lasers in Medical Science	en
dc.identifier.doi	10.1007/PL00011361	en
dc.identifier.isi	ISI:000171789000003	en
dc.identifier.volume	16	en
dc.identifier.issue	4	en
dc.identifier.spage	253	en
dc.identifier.epage	259	en