Texture analysis of fluorescence microscopic images of colonic tissue sections

Atlamazoglou, V; Yova, D; Kavantzas, N; Loukas, S

dc.contributor.author	Atlamazoglou, V	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Kavantzas, N	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T01:17:13Z
dc.date.available	2014-03-01T01:17:13Z
dc.date.issued	2001	en
dc.identifier.issn	0140-0118	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14395
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0035044127&partnerID=40&md5=b0352b9652a738b15671ae1fd7139056	en
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0035044127&partnerID=40&md5=b0352b9652a738b15671ae1fd7139056	en
dc.subject	Co-occurence matrix	en
dc.subject	Colon tissue sections	en
dc.subject	Fluorescence microscopy	en
dc.subject	Image analysis	en
dc.subject	Rhodamine derivatives	en
dc.subject	Texture analysis	en
dc.subject.classification	Computer Science, Interdisciplinary Applications	en
dc.subject.classification	Engineering, Biomedical	en
dc.subject.classification	Mathematical & Computational Biology	en
dc.subject.classification	Medical Informatics	en
dc.subject.other	Optical microscopy	en
dc.subject.other	Organic acids	en
dc.subject.other	Textures	en
dc.subject.other	Tissue	en
dc.subject.other	Fluorescence microscopy images	en
dc.subject.other	Medical imaging	en
dc.subject.other	boronic acid derivative	en
dc.subject.other	fluorescent dye	en
dc.subject.other	rhodamine B	en
dc.subject.other	article	en
dc.subject.other	calculation	en
dc.subject.other	classification	en
dc.subject.other	colon adenocarcinoma	en
dc.subject.other	colon mucosa	en
dc.subject.other	controlled study	en
dc.subject.other	decision making	en
dc.subject.other	device	en
dc.subject.other	diagnostic value	en
dc.subject.other	discriminant analysis	en
dc.subject.other	evaluation	en
dc.subject.other	fluorescence microscopy	en
dc.subject.other	human	en
dc.subject.other	human tissue	en
dc.subject.other	image analysis	en
dc.subject.other	mathematical computing	en
dc.subject.other	scoring system	en
dc.subject.other	staining	en
dc.subject.other	tissue section	en
dc.subject.other	Adenocarcinoma	en
dc.subject.other	Colon	en
dc.subject.other	Colonic Neoplasms	en
dc.subject.other	Humans	en
dc.subject.other	Image Processing, Computer-Assisted	en
dc.subject.other	Intestinal Mucosa	en
dc.subject.other	Microscopy, Fluorescence	en
dc.title	Texture analysis of fluorescence microscopic images of colonic tissue sections	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2001	en
heal.abstract	The aim of this study was to assess the potential of texture analysis for the characterization of fluorescence images from colonic tissue sections stained with a novel and selective fluoroprobe, Rhodamine B-phenylboronic acid. Fluorescence microscopy images of colonic healthy mucosa (n=35) and adenocarcinomas (n=35) were digitally captured and subjected to image texture analysis. Textural features derived from the grey level co-occurrence matrix were calculated. A modified version of the multiple discriminant analysis criterion was used to choose an appropriate subset of features. A minimum Mahalanobis distance, linear discriminant classifier and a simple evaluation 'score' method were used to classify image feature data into the two categories. A subset of four textural features was selected and used for the description and classification of each image field. They were found appropriate to correctly classify 95% of the images into the two classes, using two different classifiers. These features contained information about local homogeneity and grey level linear dependencies of the image. This study demonstrated that texture analysis techniques could provide valuable diagnostic decision support in a complex domain such as colorectal tissue.	en
heal.publisher	PETER PEREGRINUS LTD	en
heal.journalName	Medical and Biological Engineering and Computing	en
dc.identifier.isi	ISI:000168606000001	en
dc.identifier.volume	39	en
dc.identifier.issue	2	en
dc.identifier.spage	145	en
dc.identifier.epage	151	en