Production of acidic xylo-oligosaccharides by a family 10 endoxylanase from Thermoascus aurantiacus and use as plant growth regulators

Katapodis, P; Kavarnou, A; Kintzios, S; Pistola, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Kavarnou, A	en
dc.contributor.author	Kintzios, S	en
dc.contributor.author	Pistola, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:18:15Z
dc.date.available	2014-03-01T01:18:15Z
dc.date.issued	2002	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/14893
dc.subject	Acidic oligosaccharides	en
dc.subject	Plant growth regulators	en
dc.subject	Xylanase	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	oligosaccharide	en
dc.subject.other	phytohormone	en
dc.subject.other	protein hydrolysate	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	acidity	en
dc.subject.other	anion exchange	en
dc.subject.other	article	en
dc.subject.other	callus	en
dc.subject.other	carbon nuclear magnetic resonance	en
dc.subject.other	cotton	en
dc.subject.other	culture medium	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	gossypium hirsutum	en
dc.subject.other	mallow	en
dc.subject.other	malva silvestris	en
dc.subject.other	nonhuman	en
dc.subject.other	plant	en
dc.subject.other	protein family	en
dc.subject.other	protein structure	en
dc.subject.other	purification	en
dc.subject.other	solubility	en
dc.subject.other	somatic embryogenesis	en
dc.subject.other	Thermoascus	en
dc.subject.other	Gossypium	en
dc.subject.other	Gossypium hirsutum	en
dc.subject.other	Malva	en
dc.subject.other	Malva neglecta	en
dc.subject.other	Malva sylvestris	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Production of acidic xylo-oligosaccharides by a family 10 endoxylanase from Thermoascus aurantiacus and use as plant growth regulators	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1019898414801	en
heal.identifier.secondary	http://dx.doi.org/10.1023/A:1019898414801	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	Neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 10 class, from Thermoascus aurantiacus. The main acidic xylooligosaccharide (aldotetrauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the primary structure was determined by C-13 NMR spectroscopy. The aldotetrauronic yield was 15% (w/w) of the total solubilised sugars. The addition of purified aldoterauronic acid at 1.6-16 mg l(-1) growth medium, induced callus and somatic embryogenesis in culture explants of common mallow (Malva silvestris L.) and cotton (Gosssypium hirsutum).	en
heal.publisher	KLUWER ACADEMIC PUBL	en
heal.journalName	Biotechnology Letters	en
dc.identifier.doi	10.1023/A:1019898414801	en
dc.identifier.isi	ISI:000177576200006	en
dc.identifier.volume	24	en
dc.identifier.issue	17	en
dc.identifier.spage	1413	en
dc.identifier.epage	1416	en