A study on the process of lipase-catalyzed synthesis of α-pinene oxide in organic solvents

Skouridou, V; Stamatis, H; Kolisis, FN

dc.contributor.author	Skouridou, V	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Kolisis, FN	en
dc.date.accessioned	2014-03-01T01:18:34Z
dc.date.available	2014-03-01T01:18:34Z
dc.date.issued	2003	en
dc.identifier.issn	1024-2422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15093
dc.subject	α-Pinene	en
dc.subject	Candida antarctica lipase	en
dc.subject	Epoxide	en
dc.subject	Hydrogen peroxide	en
dc.subject	Peroxycarboxylic acid	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Biosynthesis	en
dc.subject.other	Carboxylic acids	en
dc.subject.other	Hydrogen peroxide	en
dc.subject.other	Lipids	en
dc.subject.other	Mixtures	en
dc.subject.other	Oxidation	en
dc.subject.other	Toluene	en
dc.subject.other	Lipase catalyzed synthesis	en
dc.subject.other	Reaction cycles	en
dc.subject.other	Reaction mixtures	en
dc.subject.other	Enzymes	en
dc.subject.other	carboxylic acid	en
dc.subject.other	epoxide	en
dc.subject.other	hydrogen peroxide	en
dc.subject.other	lipase B	en
dc.subject.other	octanoic acid derivative	en
dc.subject.other	organic solvent	en
dc.subject.other	oxide	en
dc.subject.other	pinene oxide	en
dc.subject.other	toluene	en
dc.subject.other	triacylglycerol lipase	en
dc.subject.other	unclassified drug	en
dc.subject.other	article	en
dc.subject.other	Candida antarctica	en
dc.subject.other	catalysis	en
dc.subject.other	chemical reaction kinetics	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	drug mixture	en
dc.subject.other	enzyme immobilization	en
dc.subject.other	epoxidation	en
dc.subject.other	exposure	en
dc.subject.other	nonhuman	en
dc.subject.other	oxidation	en
dc.subject.other	partial drug synthesis	en
dc.subject.other	process development	en
dc.subject.other	temperature sensitivity	en
dc.subject.other	Candida antarctica	en
dc.title	A study on the process of lipase-catalyzed synthesis of α-pinene oxide in organic solvents	en
heal.type	journalArticle	en
heal.identifier.primary	10.1080/10242420310001597801	en
heal.identifier.secondary	http://dx.doi.org/10.1080/10242420310001597801	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	The synthesis of alpha-pinene oxide was studied in a three-phase system where immobilized Candida antarctica lipase B (Novozyme 435) was used to catalyze the formation of peroxyoctanoic acid from the parent carboxylic acid and hydrogen peroxide in toluene. The peroxycarboxylic acid formed was then used in situ for the oxidation of alpha-pinene to the corresponding epoxide. When hydrogen peroxide was added in the reaction mixture gradually over 6 h, conversions increased up to 31.6%. Initial rates of alpha-pinene oxidation increased from 85 to 708 mmol L-1 h(-1) when the amount of H2O2 increased from 5 to 60 mmol. When the lipase was exposed to 75 mmol H2O2 for 0.5 h before its addition in the reaction mixture, its activity decreased to about 50%. The reusability of lipase was studied in five reaction cycles and was found to depend on the concentration of the hydrogen peroxide used.	en
heal.publisher	TAYLOR & FRANCIS LTD	en
heal.journalName	Biocatalysis and Biotransformation	en
dc.identifier.doi	10.1080/10242420310001597801	en
dc.identifier.isi	ISI:000187367000001	en
dc.identifier.volume	21	en
dc.identifier.issue	6	en
dc.identifier.spage	285	en
dc.identifier.epage	290	en