Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases

Christakopoulos, P; Katapodis, P; Kalogeris, E; Kekos, D; Macris, BJ; Stamatis, H; Skaltsa, H

dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Skaltsa, H	en
dc.date.accessioned	2014-03-01T01:18:40Z
dc.date.available	2014-03-01T01:18:40Z
dc.date.issued	2003	en
dc.identifier.issn	0141-8130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15144
dc.subject	Acidic xylo-oligosaccharides	en
dc.subject	Antimicrobial activity	en
dc.subject	Xylanases	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	10 endoxylanase	en
dc.subject.other	11 endoxylanase	en
dc.subject.other	4 o methyl dextro glucuronic acid	en
dc.subject.other	aldopentauronic acid	en
dc.subject.other	aldotetrauronic acid	en
dc.subject.other	bacterial enzyme	en
dc.subject.other	glucuronic acid	en
dc.subject.other	glucuronoxylan	en
dc.subject.other	oligosaccharide	en
dc.subject.other	unclassified drug	en
dc.subject.other	uronic acid	en
dc.subject.other	xylan	en
dc.subject.other	xylooligosaccharide	en
dc.subject.other	aerobic bacterium	en
dc.subject.other	anaerobic bacterium	en
dc.subject.other	anion exchange	en
dc.subject.other	antimicrobial activity	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	birch	en
dc.subject.other	carbohydrate analysis	en
dc.subject.other	carbohydrate synthesis	en
dc.subject.other	controlled study	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	Helicobacter pylori	en
dc.subject.other	nonhuman	en
dc.subject.other	nuclear magnetic resonance spectroscopy	en
dc.subject.other	Sporothrix	en
dc.subject.other	Thermoascus	en
dc.subject.other	Anti-Bacterial Agents	en
dc.subject.other	Chromatography	en
dc.subject.other	Chromatography, Thin Layer	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Endo-1,4-beta Xylanases	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Magnetic Resonance Spectroscopy	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Uronic Acids	en
dc.subject.other	Xylan Endo-1,3-beta-Xylosidase	en
dc.subject.other	Xylans	en
dc.subject.other	Xylosidases	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Helicobacter	en
dc.subject.other	Helicobacter pylori	en
dc.subject.other	Negibacteria	en
dc.subject.other	Posibacteria	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0141-8130(02)00079-X	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0141-8130(02)00079-X	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Acidic oligosaccharides were obtained from birchwood xylan by treatment with a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family I I endoxylanases. The main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-O-methyl-D-glucuronic acid. The xylanase from T aurantiacus liberate from glueuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from S. thermophile, which liberated an aldopentauronic acid. Acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography (SEC) and the primary structure was determined by C-13 NMR spectroscopy. The acidic xylooligosaccharides were tested against three Gram-positive and three Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori. Aldopentauronic acid was proved more active against the Gram-positive bacteria and against H. pylori. (C) 2002 Elsevier Science B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	International Journal of Biological Macromolecules	en
dc.identifier.doi	10.1016/S0141-8130(02)00079-X	en
dc.identifier.isi	ISI:000180985100007	en
dc.identifier.volume	31	en
dc.identifier.issue	4-5	en
dc.identifier.spage	171	en
dc.identifier.epage	175	en