Enzymic production of a feruloylated oligosaccharide with antioxidant activity from wheat flour arabinoxylan

Katapodis, P; Vardakou, M; Kalogeris, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Vardakou, M	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:18:57Z
dc.date.available	2014-03-01T01:18:57Z
dc.date.issued	2003	en
dc.identifier.issn	1436-6207	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15277
dc.subject	Antioxidant	en
dc.subject	Endoxylanase	en
dc.subject	Feruloyl-oligosaccharide	en
dc.subject	Low-density lipoprotein	en
dc.subject	Wheat flour arabinoxylan	en
dc.subject.classification	Nutrition & Dietetics	en
dc.subject.other	antioxidant	en
dc.subject.other	arabinose	en
dc.subject.other	arabinoxylan	en
dc.subject.other	copper intrauterine device	en
dc.subject.other	ferulic acid	en
dc.subject.other	feruloylarabinoxylotrisaccharide	en
dc.subject.other	low density lipoprotein	en
dc.subject.other	oligosaccharide	en
dc.subject.other	unclassified drug	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	xylose	en
dc.subject.other	anion exchange chromatography	en
dc.subject.other	antioxidant activity	en
dc.subject.other	article	en
dc.subject.other	carbohydrate analysis	en
dc.subject.other	carbohydrate synthesis	en
dc.subject.other	dose response	en
dc.subject.other	flour	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	hydrolysis	en
dc.subject.other	lipid oxidation	en
dc.subject.other	saponification	en
dc.subject.other	solubilization	en
dc.subject.other	spectrophotometry	en
dc.subject.other	wheat	en
dc.subject.other	Antioxidants	en
dc.subject.other	Chromatography, Gel	en
dc.subject.other	Chromatography, Ion Exchange	en
dc.subject.other	Coumaric Acids	en
dc.subject.other	Endo-1,4-beta Xylanases	en
dc.subject.other	Free Radical Scavengers	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Lipid Peroxidation	en
dc.subject.other	Lipoproteins, LDL	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Triticum	en
dc.subject.other	Xylans	en
dc.subject.other	Xylosidases	en
dc.subject.other	Hordeum vulgare subsp. vulgare	en
dc.subject.other	Poaceae	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.subject.other	Triticum aestivum	en
dc.subject.other	Zea mays	en
dc.title	Enzymic production of a feruloylated oligosaccharide with antioxidant activity from wheat flour arabinoxylan	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00394-003-0400-z	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00394-003-0400-z	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Background Main cereals such as rice, wheat, barley, and corn belong to the family Gramineae and have similar cell-wall composition. Since cereal cell walls are a good source of dietary fibre, meeting one-half of the daily requirement of 30 g of dietary fibre can be achieved by the regular consumption of cereals. Many studies have dealt with the isolation of feruloylated oligosaccharides from Gramineae by treatment with polysaccharide hydrolysing enzymes. Aim of this study Therefore, the purpose of this study was to investigate the production of feruloylated oligosaccharides from insoluble wheat flour arabinoxylan (WFAX) by treatment with a Thermoascus aurantiacus family 10 endoxylanase (XYLI) and the evaluation of their antioxidant activity. Methods The main feruloylated oligosaccharide was purified by anion-exchange and size-exclusion chromatography (SEC). Alkaline saponification and acid hydrolysis were used for product identification. Evaluation of antioxidant activity was performed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) reduction assay and the inhibition of copper-mediated oxidation of low density lipoprotein (LDL). Results The optimal conditions for WFAX hydrolysis using the XYLI have been determined to be 100 U g(-1) of WFAX for 30 min at 50 degreesC. Saponification of the oligosaccharide released FA and oligosaccharide. The released oligosaccharide consisted of arabinose and xylose in a molar ratio of 1:3 and these results support the identity of the feruloylated oligosaccharide as feruloyl arabinoxylotrisaccharide (FAX(3)). FAX(3) showed profound antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) reduction assay exhibiting an antiradical efficiency of 0.035 (x 10(-3)) and inhibited the copper-mediated oxidation of human low density lipoprotein (LDL) in a dose-dependent manner with almost complete inhibition at 32 muM. Conclusion A feruloylated oligosaccharide (FAX(3)) was isolated from WFAX after enzymatic treatment with XYLI. We verified antioxidant activity of FAX(3) which may be important in preventing or reducing the progression of atherosclerosis by inhibiting the peroxidation of lipoproteins.	en
heal.publisher	DR DIETRICH STEINKOPFF VERLAG	en
heal.journalName	European Journal of Nutrition	en
dc.identifier.doi	10.1007/s00394-003-0400-z	en
dc.identifier.isi	ISI:000181514100008	en
dc.identifier.volume	42	en
dc.identifier.issue	1	en
dc.identifier.spage	55	en
dc.identifier.epage	60	en