Induction, purification, and characterization of two extracellular α-L-arabinofuranosidases from Fusarium oxysporum

Panagiotou, G; Topakas, E; Economou, L; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Economou, L	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:03Z
dc.date.available	2014-03-01T01:19:03Z
dc.date.issued	2003	en
dc.identifier.issn	0008-4166	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15343
dc.subject	α-L-arabinofuranosidase	en
dc.subject	Enzyme induction	en
dc.subject	Enzyme purification	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Immunology	en
dc.subject.classification	Microbiology	en
dc.subject.other	Enzymes	en
dc.subject.other	Esters	en
dc.subject.other	pH effects	en
dc.subject.other	Phenols	en
dc.subject.other	Solubility	en
dc.subject.other	Enzyme purification	en
dc.subject.other	Molecular masses	en
dc.subject.other	Microbiology	en
dc.subject.other	4 nitrophenyl alpha arabinofuranoside	en
dc.subject.other	alpha arabinofuranosidase	en
dc.subject.other	arabinose	en
dc.subject.other	arabinoxylan	en
dc.subject.other	benzene derivative	en
dc.subject.other	carbon	en
dc.subject.other	ferulic acid	en
dc.subject.other	fungal enzyme	en
dc.subject.other	phenol derivative	en
dc.subject.other	protein subunit	en
dc.subject.other	unclassified drug	en
dc.subject.other	enzyme	en
dc.subject.other	fungus	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme analysis	en
dc.subject.other	enzyme induction	en
dc.subject.other	enzyme purification	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Michaelis constant	en
dc.subject.other	molecular weight	en
dc.subject.other	nonhuman	en
dc.subject.other	pH	en
dc.subject.other	priority journal	en
dc.subject.other	stereospecificity	en
dc.subject.other	sugar beet	en
dc.subject.other	Arabinose	en
dc.subject.other	Biomass	en
dc.subject.other	Culture Media	en
dc.subject.other	Enzyme Induction	en
dc.subject.other	Fusarium	en
dc.subject.other	Glycoside Hydrolases	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Substrate Specificity	en
dc.subject.other	Beta vulgaris subsp. vulgaris	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Triticum aestivum	en
dc.title	Induction, purification, and characterization of two extracellular α-L-arabinofuranosidases from Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1139/w03-077	en
heal.identifier.secondary	http://dx.doi.org/10.1139/w03-077	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	In the presence of L-arabinose as sole carbon source, Fusarium oxysporum produces two alpha-L-arabinofuranosidases (ABFs) named ABF1 and ABF2, with molecular masses of 200 and 180 kDa, respectively. The two F. oxysporum proteins have been purified to homogeneity. The purified enzymes are composed of three equal subunits and are neutral proteins with pIs of 6.0 and 7.3 for ABF1 and ABF2, respectively. With p-nitrophenyl alpha-L-arabinofuranoside (pNPA) as the substrate, ABF1 and ABF2 exhibited K-m values of 0.39 and 0.28 mmol.L-1, respectively, and V-max values of 1.6 and 4.6 mumol.min(-1).(mg of protein)(-1), respectively, and displayed optimal activity at pH 6.0 and 50-60degreesC. ABFs released arabinose only from sugar beet arabinan and not from wheat soluble and insoluble arabinoxylans. The enzymes were not active on substrates containing ferulic acid ester linked to C-5 and C-2 linkages of pNPA showing that phenolic substituents of pNPA sterically hindered the action of ABFs.	en
heal.publisher	NATL RESEARCH COUNCIL CANADA	en
heal.journalName	Canadian Journal of Microbiology	en
dc.identifier.doi	10.1139/w03-077	en
dc.identifier.isi	ISI:000187527100007	en
dc.identifier.volume	49	en
dc.identifier.issue	10	en
dc.identifier.spage	639	en
dc.identifier.epage	644	en