Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan

Vardakou, M; Katapodis, P; Samiotaki, M; Kekos, D; Panayotou, G; Christakopoulos, P

dc.contributor.author	Vardakou, M	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Samiotaki, M	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Panayotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:17Z
dc.date.available	2014-03-01T01:19:17Z
dc.date.issued	2003	en
dc.identifier.issn	0141-8130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15400
dc.subject	Arabinoxylan	en
dc.subject	Endoxylanase	en
dc.subject	Enzyme family	en
dc.subject	Mode of action	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	arabinoxylan	en
dc.subject.other	disaccharide	en
dc.subject.other	feruloyl arabinoxydisaccharide	en
dc.subject.other	oligosaccharide	en
dc.subject.other	unclassified drug	en
dc.subject.other	water	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	anion exchange	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	hydrolysis	en
dc.subject.other	incubation time	en
dc.subject.other	molecular size	en
dc.subject.other	nonhuman	en
dc.subject.other	Sporothrix	en
dc.subject.other	Thermoascus	en
dc.subject.other	Biochemistry	en
dc.subject.other	Chromatography, Gel	en
dc.subject.other	Chromatography, Ion Exchange	en
dc.subject.other	Coumaric Acids	en
dc.subject.other	Endo-1,4-beta Xylanases	en
dc.subject.other	Eurotiales	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Spectrophotometry	en
dc.subject.other	Sporothrix	en
dc.subject.other	Water	en
dc.subject.other	Xylans	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0141-8130(03)00077-1	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0141-8130(03)00077-1	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Microbial endo-beta-1,4-xylanases(EXs,EC3.2.1.8) belonging to glycanase families 10 and 11 differ in their action on water-unextractable arabinoxylan (WU-AX). WU-AX was incubated with different levels of a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family 11 endoxylanases. At 10 g l(-1) arabinoxylan, enzyme concentrations (K-E values) needed to obtain half-maximal hydrolysis rates (V-max values) were 4.4 nM for the xylanase from T aurantiacus and 7.1 nM for the xylanase from S. thermophile. Determination of V-max/K-E revealed that the family 10 enzyme hydrolysed two times more efficiently WU-AX than the family 11 enzyme. Molecular weights of the products formed were assessed and separation of feruloyl-oligosaccharides was achieved by anion-exchange and size-exclusion chromatography (SEC). The main difference between the feruloylated products by xylanases of family 10 and 11 concerned the length of the products containing feruloyl-arabinosyl substitution. The xylanase from T aurantiacus liberated from WU-AX a feruloyl arabinoxylodisaccharide (FAX(2)) as the shortest feruloylated fragment in contrast with the enzyme from S. thermophile, which liberated a feruloyl arabinoxylotrisaccharide (FAX(3)). These results indicated that different factors govern WU-AX breakdown by the two endoxylanases. (C) 2003 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	International Journal of Biological Macromolecules	en
dc.identifier.doi	10.1016/S0141-8130(03)00077-1	en
dc.identifier.isi	ISI:000186835800019	en
dc.identifier.volume	33	en
dc.identifier.issue	1-3	en
dc.identifier.spage	129	en
dc.identifier.epage	134	en