Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes

Kalogeris, E; Christakopoulos, P; Katapodis, P; Alexiou, A; Vlachou, S; Kekos, D; Macris, BJ

dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Alexiou, A	en
dc.contributor.author	Vlachou, S	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:19:26Z
dc.date.available	2014-03-01T01:19:26Z
dc.date.issued	2003	en
dc.identifier.issn	0032-9592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15498
dc.subject	β-glucosidase	en
dc.subject	Cellulose hydrolysis	en
dc.subject	Endoglucanase	en
dc.subject	Solid state cultivation	en
dc.subject	Thermoascus aurantiacus	en
dc.subject	Thermophilic fungus	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Engineering, Chemical	en
dc.subject.other	Fungi	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0032-9592(02)00242-X	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0032-9592(02)00242-X	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Extracellular cellulolytic enzymes were produced under solid state cultivation by the thermophilic fungus Thermoascus aurantiacus and characterized. Elevated levels of endoglucanase and beta-glucosidase activities were produced simultaneously by optimization of growth factors. Under optimal growth conditions, 1572 U endoglucanase and 101.6 U beta-glucosidase per g of carbon source were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl-beta-glycosides of glucose (MUG) and cellobiose (MUG(2)) were used to characterize the cellulolytic multienzyme components after separation by isoelectric focusing. The zymogram indicated one endoglucanase and one beta-glucosidase with pI values 3.5 and 3.9, respectively. Both enzymes exhibited significant thermostability, with half-lives of 42 and 18 min, respectively, at 80 degreesC. (C) 2002 Elsevier Science Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Process Biochemistry	en
dc.identifier.doi	10.1016/S0032-9592(02)00242-X	en
dc.identifier.isi	ISI:000183135000015	en
dc.identifier.volume	38	en
dc.identifier.issue	7	en
dc.identifier.spage	1099	en
dc.identifier.epage	1104	en