Production and partial characterisation of feruloyl esterase by Sporotrichum thermophile in solid-state fermentation

Topakas, E; Kalogeris, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:26Z
dc.date.available	2014-03-01T01:19:26Z
dc.date.issued	2003	en
dc.identifier.issn	0032-9592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15499
dc.subject	Feruloyl esterases	en
dc.subject	Solid state culture	en
dc.subject	Sporotrichum thermophile	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Engineering, Chemical	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fungi	en
dc.subject.other	Sporothrix	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Triticum aestivum	en
dc.title	Production and partial characterisation of feruloyl esterase by Sporotrichum thermophile in solid-state fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0032-9592(03)00044-X	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0032-9592(03)00044-X	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	A number of factors affecting production of feruloyl esterase an enzyme that hydrolyse ester linkages of ferulic acid (FA) in plant cell walls, by the thermophylic fungus Sporotrichum thermophile under solid state fermentation (SSF) were investigated. Initial moisture content and type of carbon source were consecutively optimised. SSF in a laboratory horizontal bioreactor using the optimised medium allowed the production of 156 mU g(-1) of carbon source, which compared favourably with those reported for the other micro-organisms. Optimal esterase activity was observed at pH 8 and 60 degreesC. The activity of the esterase was measured on an insoluble feruloylated hemicellulose substrate (de-starched wheat bran (DSWB)). De-esterifcation of wheat straw yielded loss of feruloyl esterase production even though the supplementation of free FA comparable to the alkali-extractable levels of FA found in wheat straw. Chromogenic (fluorogenic) 4-methylumbelliferyl ferulate was used to characterise the multienzyme component, after separation by isoelectric focusing and native PAGE electrophoresis. The zymograms indicated one major esterase activity exhibiting pI and molecular mass values 5 and 27 kDa, respectively. (C) 2003 Elsevier Science Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Process Biochemistry	en
dc.identifier.doi	10.1016/S0032-9592(03)00044-X	en
dc.identifier.isi	ISI:000184064300004	en
dc.identifier.volume	38	en
dc.identifier.issue	11	en
dc.identifier.spage	1539	en
dc.identifier.epage	1543	en