Production and partial characterization of xylanase by Sporotrichum thermophile under solid-state fermentation

Topakas, E; Katapodis, P; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:27Z
dc.date.available	2014-03-01T01:19:27Z
dc.date.issued	2003	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15500
dc.subject	Solid state culture	en
dc.subject	Sporotrichum thermophile	en
dc.subject	Xylanase	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fungi	en
dc.subject.other	Sporothrix	en
dc.subject.other	Sporotrichum	en
dc.title	Production and partial characterization of xylanase by Sporotrichum thermophile under solid-state fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1023207429410	en
heal.identifier.secondary	http://dx.doi.org/10.1023/A:1023207429410	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	A number of factors affecting production of xylanase, by the thermophilic fungus Sporotrichum thermophile under solid state fermentation (SSF) were investigated. Initial moisture content and type of carbon source were consecutively optimized. Solid state fermentation in a laboratory horizontal bioreactor using the optimized medium allowed the production of 320 U g(-1) of carbon source which compared favourably with those reported for other microorganisms. Optimal xylanase activity was observed at pH 5 and 70degreesC. Chromogenic (fluorogenic) 4-methylumbelliferyl beta-glycoside of xylobiose (MUX2) was used to characterize the xylanase multienzyme component, after separation by isoelectric focusing and native PAGE electrophoresis. The zymograms indicated one major xylanase fraction exhibiting pI and molecular mass values 4 and 90-120 kDa, respectively.	en
heal.publisher	KLUWER ACADEMIC PUBL	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1023/A:1023207429410	en
dc.identifier.isi	ISI:000182132100015	en
dc.identifier.volume	19	en
dc.identifier.issue	2	en
dc.identifier.spage	195	en
dc.identifier.epage	198	en