Production of β-fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides

Katapodis, P; Kalogeris, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:27Z
dc.date.available	2014-03-01T01:19:27Z
dc.date.issued	2003	en
dc.identifier.issn	0890-5436	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15501
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	Catalyst activity	en
dc.subject.other	Fungi	en
dc.subject.other	Hydrolysis	en
dc.subject.other	pH effects	en
dc.subject.other	Sugar (sucrose)	en
dc.subject.other	Synthesis (chemical)	en
dc.subject.other	Thermophilic fungi	en
dc.subject.other	Enzymes	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fungi	en
dc.subject.other	Sporothrix	en
dc.subject.other	Sporotrichum	en
dc.title	Production of β-fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides	en
heal.type	journalArticle	en
heal.identifier.primary	10.1081/FBT-120019980	en
heal.identifier.secondary	http://dx.doi.org/10.1081/FBT-120019980	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Production of beta-fructofuranosidase from the thermophilic fungus Sporotrichum thermophile was studied. The effect of nitrogen source, as well as the type and concentration of carbon source on enzyme production was examined. The results from flask experiments were used for the production of the enzyme in 7-1 bioreactors. beta-Fructofuranosidase from Sporotrichum thermophile showed both transfructosylating and hydrolytic activities. It was optimally, active at 60degreesC, while the optimal pHs for hydrolysis and transfructosylation were 4.0 and 6.0, respectively. Synthesis of fructooligosaccharides was maximized at 20% (w/v) initial sucrose concentration. The major sugar produced by the transfructosylating activity of the enzyme was 6-kestose.	en
heal.publisher	MARCEL DEKKER INC	en
heal.journalName	Food Biotechnology	en
dc.identifier.doi	10.1081/FBT-120019980	en
dc.identifier.isi	ISI:000182489300001	en
dc.identifier.volume	17	en
dc.identifier.issue	1	en
dc.identifier.spage	1	en
dc.identifier.epage	14	en